Background 2,3‐butanediol is a chemical component with wide industrial applications, such as biofuels and chemical precursors. Reducing 2,3‐butanediol production costs is a high priority and recent focus has been on its biological synthesis from raw renewable materials rather than through conventional chemical procedures. In this study, a 2,3‐butanediol native bacterial producer was selected for the fermentation of Agave cupreata bagasse waste by enzymatic hydrolysation to obtain 2,3‐butanediol. Results The native Klebsiella oxytoca UM2‐17 strain was selected to efficiently produce 2,3‐butanediol on synthetic mineral media. Using glucose (100 g L−1) and glucose: xylose (50 g L−1:50 g L−1) as sugar sources obtained titres of 29.36 g L−1 and 25.9 g L−1 of 2,3‐butanediol, corresponding to yields of 0.29 g g−1 and 0.26 g g−1, respectively. Agave bagasse solid waste after acid pre‐treatment was utilized to obtain enzymatic hydrolysates, obtaining liquors with glucose/xylose/arabinose at 25.8 g L−1 with efficiency of ∼52.5% sugar conversion from residual solid pre‐hydrolysed bagasse. The production of 2,3‐butanediol through batch fermentation of the enzymatic‐hydrolysate by the UM2‐17 strain showed total depletion of glucose and xylose, achieving a maximal production of 10.3 g L−1 of 2,3‐butanediol plus 0.28 g L−1 ethanol, corresponding to 2,3‐butanediol yield of 0.40 g g−1. Conclusion The results indicate that A. cupreata bagasse waste from mezcal beverage elaboration is a sustainable bioresource for production of 2,3‐butanediol by fermentation of enzymatic bagasse‐hydrolysate using the K. oxytoca UM2‐17 strain. © 2019 Society of Chemical Industry
La contaminación del suelo por aceite residual automotriz (ARA) que es una mezcla de hidrocarburos (HC), que impide la actividad microbiana del reciclaje de elementos necesarios para la vida y afecta negativamente su fertilidad. La bioestimulación (BIS) es una estrategia para eliminar el ARA a un nivel que permite la recuperación de su fertilidad a un valor inferior al máximo de 4400 ppm de la NOM-138-SEMARNAT/SSA1-2012 (NOM-138). El objetivo de este trabajo fue la BIS un suelo impactado por 17000 ppm de ARA por enriquecimiento independiente: con solución mineral (SM), lombricomposta (LC) y P. vulgaris o abono verde (AV). La recuperación del suelo se determinó mediante la: liberación de CO 2 por oxidación del ARA, por la población bacteriana oxidante de ARA (BOARA) y la concentración de ARA por Soxhlet, los datos experimentales se analizaron con Tukey-error estándar. Los resultados revelaron que la BIS de suelo impactado por 17000 ppm de ARA con la SM lo decreció hasta 4386 ppm, en tanto que con la LC lo disminuyo a 3766 ppm en 6 meses, ambos valores numéricos inferiores al máximo aceptado por la NOM-13, estadísticamente diferentes con los 8596 ppm en suelo bioestimulado mediante P. vulgaris o AV y los 16000 ppm de ARA en el suelo sin bioestimular o control negativo. Se concluye que la BIS, es específica acorde a la complejidad de los HC como el ARA, que se eliminó con la SM y la LC según la NOM-138 para la recuperación de la fertilidad del suelo. 2020. Journal of the Selva Andina Research Society®. Bolivia. Todos los derechos reservados.
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