Peptides derived from protein tandem mass spectrometry data have been mapped to the human genome sequence forming an expandable resource for the proteomic data.

High performance liquid chromatography coupled with microdialysis sampling and electrochemical detection (HPLC-ECD) has been used to determine dopamine (DA). In the HPLC-ECD a multi-wall carbon nanotube electrode chemically modified with carboxyl groups (MWNT-COOH CME) was used as the working electrode for determination of DA. The results indicated that the MWNT-COOH CME enabled efficient electrocatalytic oxidation of DA with relatively high sensitivity and stability and long life. Peak currents for dopamine were linearly dependent on concentration in the range 5.0 x 10(-9) to 5.0 x 10(-5) mol L(-1) and the calculated detection limit (S/N=3) was 2.5 x 10(-9) mol L(-1). The method had been successfully used to measure dopamine in rat striatal microdialysate. To study the physiological effect of nitric oxide (NO) on striatal release of DA, 0.5 mmol L(-1) sodium nitroprusside (SNP) was a continuously perfused into rat striatum. This resulted in a 46% increase in DA basal level.

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A label-free ECL aptamer biosensor based on CdS QDs/ACNTs electrode for the sensitive detection of thrombin was fabricated. CdS QDs and Chitosan (CTS) complex films were coated and filled the tubes of ACNTs by carrying out electrodeposition reaction of CTS-CdS QDs on ACNTs electrode, the resulting electrode showed high ECL intensity and good biocompatibility. After aptamer was bound to the film via glutaric dialdehyde, the modified electrode could be used as an ECL aptamer sensor for the thrombin detection. The specific reaction between thrombin and aptamer resulted in the decrease in ECL intensity. The change of the ECL intensity was found to be linear with the logarithm of thrombin concentration in the range from 1.0 10 À13 M to 1.0 10 À9 M. The ECL aptamer sensor had the advantages of speed, high sensitivity, specificity and stability, which could be a promising technique for protein detection.