Electrochemiluminescence Aptamer Biosensor for Detection of Thrombin Based on CdS QDs/ACNTs Electrode

Yang, Lizhu; Zhu, Jing; Xu, Ying; Yun, Wen; Zhang, Renyi; He, Pingang; Fang, Yuzhi

doi:10.1002/elan.201000404

Cited by 30 publications

(18 citation statements)

References 42 publications

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“…He et al also fabricated a label-free ECL aptamer biosensor based on CdS QD-carbon nanotube electrodes for the sensitive detection of thrombin. 83 After thrombin recognition on the aptamer modified QDs electrode, an obvious decrease in ECL signal was obtained. In addition, it has been found that the ECL of CdTe QDs displayed a size-dependent property (see Fig.…”

Section: Electrochemical and Electrochemiluminescence Sensorsmentioning

confidence: 99%

Recent advances and applications in QDs-based sensors

2011

Analyst

123

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As a unique nanomaterial, quantum dots (QDs) are not only applied in fluorescent labeling and biological imaging, but are also utilized in novel sensing systems. Because QDs have attractive optoelectronic characteristics, QD-based sensors present high sensitivity in detecting specific analytes in the chemical and biochemical fields. In this review, we describe the basic principles and different conjugation strategies in QD-based sensors. An overview of recent advances and various models of QD-sensing systems is also provided. Furthermore, perspectives for sensors based on QDs are discussed.

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Section: Electrochemical and Electrochemiluminescence Sensorsmentioning

confidence: 99%

Recent advances and applications in QDs-based sensors

2011

Analyst

123

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“…Therefore, it can be concluded that the obvious ECL decrease for thrombin solution was mostly attributed to the right recognition of aptamer to target protein. We think that the decrease in the controlling experiments came from the non-special adsorption onto electrode surface, and the adsorbed materials then acted as the electronic transfer blocker and decreased ECL intensity [46].…”

Section: The Sensitivity and Selectivity Evaluation For The Ecl Biosementioning

confidence: 99%

Solid-state electrochemiluminescence protein biosensor with aptamer substitution strategy

Dong

Zhang

et al. 2011

Sci. China Chem.

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One solid-state electrochemiluminescence (ECL) protein biosensor based on the competing reaction and substitute reaction between protein-to-DNA aptamer and DNA-to-DNA aptamer was proposed. Additionally, the biosensor was based on ECL photo-quenching effect of ferrocene (Fc) to tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ). It was built up by modification of Au nanoparticles (AuNPs) and Ru(bpy) 3 2+ on one Au electrode firstly, and then self-assembly of one special double-stranded DNA (dsDNA) onto the electrode. This dsDNA was prepared by hybridization of one Fc labeled molecular beacon single-stranded DNA(ssDNA) and one anti-thrombin aptamer ssDNA. Without the target protein, this Fc-dsDNA/Ru(bpy) 3 2+-AuNPs/Au electrode trigged strong ECL signal, so we called it ECL "signal on" state. When thrombin was present in the sensing solution, the protein reacted with its aptamer from the Fc-dsDNA/Ru(bpy) 3 2+ -AuNPs/Au electrode. Then the left molecular beacon ssDNA on the electrode recovered to its normal stem-loop structure and consequently its Fc labeler was close enough to the electrode surface to quench the ECL signal from Ru(bpy) 3 2+. It was in ECL "signal off" state. We measured the decrease in ECL intensity to sense the target protein. This was one endeavour to sense protein by using un-labeling target or probe strategy, which gave higher sensitivity and selectivity due to the better combination efficiency of protein and the un-labeled aptamer. 6.25 fmol/L thrombin was detected out. electrochemiluminescence biosensor, aptamer, tris(2,2′-bipyridyl)ruthenium(II)

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“…For example, Zhu’s group developed a series of aptasensors with the QDs ECL as the signal transduction for the detection of lysozyme, ATP and thrombin [ 58 , 59 , 60 ]. Aptasensors detecting lysozyme and ATP were built with the specific affinity between aptamer and target, on the basis of Watson–Crick base pairing.…”

Section: Aptamer-modified Qds For Optical Detectionmentioning

confidence: 99%

“…Aptasensors detecting lysozyme and ATP were built with the specific affinity between aptamer and target, on the basis of Watson–Crick base pairing. Taking lysozyme detection for example [ 60 ], thiolated anti-lysozyme aptamer were modified onto the pretreated Au electrode via an Au-S bond, and then incubated with lysozyme solution to form the aptamer-lysozyme bioaffinity complexes. The free aptamer were hybridized with biotin modified complementary DNA (biotin-cDNA) oligonucleotides.…”

Section: Aptamer-modified Qds For Optical Detectionmentioning

confidence: 99%

Aptamer-Modified Semiconductor Quantum Dots for Biosensing Applications

Wen

Qiu

et al. 2017

Sensors

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Semiconductor quantum dots have attracted extensive interest in the biosensing area because of their properties, such as narrow and symmetric emission with tunable colors, high quantum yield, high stability and controllable morphology. The introduction of various reactive functional groups on the surface of semiconductor quantum dots allows one to conjugate a spectrum of ligands, antibodies, peptides, or nucleic acids for broader and smarter applications. Among these ligands, aptamers exhibit many advantages including small size, high chemical stability, simple synthesis with high batch-to-batch consistency and convenient modification. More importantly, it is easy to introduce nucleic acid amplification strategies and/or nanomaterials to improve the sensitivity of aptamer-based sensing systems. Therefore, the combination of semiconductor quantum dots and aptamers brings more opportunities in bioanalysis. Here we summarize recent advances on aptamer-functionalized semiconductor quantum dots in biosensing applications. Firstly, we discuss the properties and structure of semiconductor quantum dots and aptamers. Then, the applications of biosensors based on aptamer-modified semiconductor quantum dots by different signal transducing mechanisms, including optical, electrochemical and electrogenerated chemiluminescence approaches, is discussed. Finally, our perspectives on the challenges and opportunities in this promising field are provided.

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Electrochemiluminescence Aptamer Biosensor for Detection of Thrombin Based on CdS QDs/ACNTs Electrode

Cited by 30 publications

References 42 publications

Recent advances and applications in QDs-based sensors

Recent advances and applications in QDs-based sensors

Solid-state electrochemiluminescence protein biosensor with aptamer substitution strategy

Aptamer-Modified Semiconductor Quantum Dots for Biosensing Applications

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