This study examines the phenomena of the hormone-active fibers obtaining process, in the form of artificial insulin depot. As a fibrous carrier of insulin cation-exchange polyacrylonitrile (PAN) fibers and biodegradable polysaccharide alginate fibers were used. The process of obtaining fibrous artificial insulin depot was based on the chemisorption of insulin from insulin aqueous solutions by these fibers. The parameters of insulin chemisorption reaction were determined and their influence on quantities of bonded insulin in the artificial depot was studied. The impact of fiber polymer nature on the intensity of insulin chemisorption was studied and determined. Also, the location and deposition of insulin in and onto the fiber, fiber topography were studied. The maximum amounts of bounded insulin for the cation-exchange PAN fibers were 395.0 mg porcine insulin chromatographic / g of fiber, and for the alginate fibers were about 300 mg of porcine insulin chromatographic / g of fiber.