This manuscript instrumented two light-duty passenger cars to construct real-world driving cycles for the Baghdad-Basrah highway road in Iraq using a data logger. The recorded data is conducted to obtain typical speed profiles for each vehicle. Each of the recruited vehicles is modelized using Advanced Vehicle Simulator and conducted on the associated created driving cycle to investigate fuel economy and analyze performance. Moreover, to inspect the influence of driving behavior on fuel consumption and emissions, the simulation process is re-implemented by substituting the conducted real-world driving cycle. The analyses are done for the first and second stages of simulation predictions to explore the fuel-penalty of aggressive driving behavior. The analysis for substitution predictions showed that fuel consumption could be reduced by 12.8% due to conducting vehicle under the more consistent real-world driving cycle. However, conducting vehicle under the more aggressive one would increase fuel consumption by 14.6%. The associated emissions change prediction due to the substitution is also achieved and presented.