The aim of this study was to investigate the possible protective role of vitamin A, C, and E on aflatoxin B(1)-induced in human lymphocytes using biochemical approaches. The control group received dimethyl sulfoxide, the second group of cultures were administered aflatoxin B(1) (AFB(1)) at a dose of 5 muM. The other group of cultures were treated with AFB(1)+vitamin A (0.5 and 1.0 and 1.5 microM) and AFB(1)+vitamin C (25, 50, and 100 microM) and AFB(1)+vitamin E (40, 100, and 200 microM). The results of this experiment show that AFB(1) significantly decreased the level of GSH and the activities of superoxide dismutase and GPx and increased level of malondialdehyde. Simultaneous supplementation with vitamin A, C, and E restored these parameters to that of normal range. In conclusion, vitamin A, C, and E exhibited protective effects in human lymphocytes by inhibiting AFB(1)-induced ROS generation.

No abstract

No abstract

No abstract

In this study, the antigenotoxic and antioxidant effects of Cetraria islandica methanol (CME) extract were determined by using sister chromatid exchange (SCE), micronuclei (MN) assays and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities and malondialdehyde (MDA) levels against effects of aflatoxin B1 (AFB₁) induced oxidative stress and genotoxicity in human lymphocytes in vitro. The results showed that the frequencies of SCE, MN and MDA level decreased, SOD and GPx activities increased when 5 μg/mL and 10 μg/mL doses of CME were added to AFB₁-treated cultures. Also, the present results indicate that CME has strong antioxidative and the antigenotoxicity mechanisms of CME are associated with its antioxidant nature.