The study included 70 Surface swabs collected from patients in different ages of both genders suffering from burns infections. All the specimens were diagnosed by subculturing in differential and selective media, microscopically and by following the biochemical tests to confirm identifications. Antibiotic susceptibility test was done to all the bacterial isolates.The identification of the clinical isolates showed that the 16(22.9%) were belonged to Staphylococcus aureus and 6(8.6%) of Staphylococcus epidermidis , whereas 23(32.8%),20(28.6%) isolates were for Pseudomonas aeruginosa ,Acinetobacter baumannii respectively. Copper oxide nanoparticles were biosynthesized by Lantana camara flower extracts which acted as a reducing agent for copper nitrate salts, characterization of the metal oxide was done by using Ultra violate visible light Spectroscopy, Fourier transforms Infrared spectroscopy, X-ray Diffraction, Atomic Force Microscopy and Field Emission Scanning Electron Microscopy. The biological activity of Copper Oxide Nanoparticles was screened toward three clinical isolates that showed multiple drug resistance isolated from burns by following agar wells diffusion method which proved the ability of copper oxide nanoparticles to inhibit growth of the clinical bacterial isolates. The minimum inhibitory concentrations were determined toward the tested bacteria by using the microdilution method.

Clinical samples 200 were collected from patients hospitalized in Baquba Teaching Hospital / Diyala during September 2021 to the end of January 2022. The clinical samples were distributed into 145 (72%) of wounds and 55 (27.5%) of burn samples. The samples were sub cultured on Blood agar Macckonky agar and Mannitol salt agar, isolated were examined microscopically and diagnosed by using biochemical tests which showed, Staphylococcus aureus 30 (17.6%) and Staphylococcus epidermidis 13 (7.64%) and Acinetobacter baumannii 56 (32.9%)