This study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL). The PDL cells were isolated from 45 extracted teeth randomly distributed among 6 groups: 5 min, 1 h, 2 h, PRF 30 min, PRF 1 h and PRF 2 h. In the groups 5 min, 1 h and 2 h (n = 5), the teeth were kept dry in extra-alveolar times of 5 min, 1 h and 2 h respectively. The teeth of the groups PRF 30 min, PRF 1 h and PRF 2 h (n = 10) were kept dry at extra-alveolar times of 30 min, 1 and 2 h followed by immersion in PRF for 45 min. PDL cells were isolated by enzymatic digestion with type II collagenase and dispase, counted and analyzed for viability with Trypan blue vital dye in Neubauer chamber. The variables total number of cells and cell viability demonstrated that in the 5 min, 1 h and 2 h groups there was a decrease after the extra-alveolar dry times of 1 and 2 h. In comparison with the total number of cells, group 1 h, considered immediate reimplantation, did not present statistical difference when compared to the groups PRF 30 min, PRF 1 h and 2 h, a result that demonstrates that PRF assists in cell maintenance and recovery. PRF provided increased cell viability in relation to the different dry extra-alveolar times analyzed (p < 0.001). Autologous PRF presented effectiveness in maintaining and recovering PDL cells from extracted teeth and kept dry for up to 2 h.

Objective: The objective of this article is to present to the dental professionals, through a literature review, the main oral lesions that are part of the odontopediatric stomatology, in order to improve the knowledge and attention to the health of the pediatric patients. Methods: The searches were carried out in the following bibliographic databases - PubMed, Web of Science, LILACS and Bireme. Data synthesis: This article deals with the description of the most frequent oral lesions in Pediatric Dentistry, ranging from characteristic changes in the newborn to pathological, non-pathological, congenital, fluid retained, ulcerative and those related to the main infectious diseases of childhood, with clinical manifestations in the oral cavity. Conclusion: It is very important, for General Practitioner and Pediatric Dentistry to,recognize oral lesions in children, to make a correct and early diagnosis of them, in order to improve the knowledge, attention to the health and quality of life of the pediatric patients. RESUMO Objetivo: apresentar aos profissionais da Odontologia, por meio de uma revisão da literatura, as principais lesões bucais que fazem parte da Odontopediatria, a fim de melhorar o conhecimento e a atenção à saúde dos pacientes pediátricos. Métodos: As buscas foram realizadas nas seguintes bases de dados bibliográficas - PubMed, Web of Science, LILACS e Bireme. Síntese dos dados: Este artigo trata da descrição das lesões bucais mais frequentes na Odontopediatria, desde mudanças características no recém-nascido até patológicas, não patológicas, congênitas, lesões com retenção de líquido, ulcerativas e aquelas relacionadas às principais doenças infecciosas da infância, com manifestações clínicas na cavidade bucal. Conclusão: É muito importante, para o clínico geral e para o odontopediatra, reconhecer as diversas lesões que podem acometer a cavidade bucal das crianças, propiciando um diagnóstico correto e precoce das mesmas, a fim de melhorar o conhecimento, atenção à saúde e qualidade de vida dos pacientes pediátricos. .