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Molecular methodologies providing data on viral concentration and infectivity have been successfully used in environmental virology, supporting quantitative risk assessment studies. The present study aimed to assess human mastadenovirus (HAdV) intact particles using a derivative of propidium monoazide associated with qPCR (PMAxx-qPCR) in aquatic matrices. Initially, different concentrations of PMAxx were evaluated to establish an optimal protocol for treating different naturally contaminated matrices, using 10 min incubation in the dark at 200 rpm at room temperature and 15 min of photoactivation in the PMA-Lite™ LED photolysis device. There was no significant reduction in the quantification of infectious HAdV with increasing concentration of PMAxx used (20 μM, 50 μM, and 100 μM), except for sewage samples. In this matrix, a reduction of 5.01 log of genomic copies (GC)/L was observed from the concentration of 50 μM and revealed 100% HAdV particles with damaged capsids. On the other hand, the mean reduction of 0.51 log in stool samples using the same concentration mentioned above demonstrated 83% of damaged particles eliminated in the stool. Following, 50 μM PMAxx-qPCR protocol revealed a log reduction of 0.91, 0.67, and 1.05 in other samples of raw sewage, brackish, and seawater where HAdV concentration reached 1.47 × 10 4 , 6.81 × 10 2 , and 2.33 × 10 2 GC/L, respectively. Fifty micrometers of PMAxx protocol helped screen intact viruses from different matrices, including sea and brackish water.

Wastewater-based monitoring has been described as a non-invasive approach to assess virus distribution in a specific geographic area. In this study we assess the genetic diversity and concentration of human adenovirus (HAdV) and human polyomavirus JC (JCPyV) in wastewater samples in Rio de Janeiro, Brazil, during the Olympic Games, Rio 2016.Wastewater samples (50 mL) obtained from domestic and hospital sewages were concentrated by the skimmed milk flocculation method and processed using molecular tools. Quantitative polymerase chain reaction using the ABI PRISM 7500 Real Time TaqMan System and TaqMan Universal Master Mix

Biotechnology is known as the set of processes and techniques that involves the manipulation of living beings, resulting in the production