The 5-methylcytosine (m5C) RNA methyltransferase NOP2/Sun RNA methyltransferase 5 (NSUN5) has been reported to serve important roles in numerous diseases. However, the functions and clinical significance of NSUN5 in hepatocellular carcinoma (HCC) remain unknown. Clinical information and NSUN5 mRNA sequencing data for 374 patients with HCC were downloaded from The Cancer Genome Atlas (TCGA) database, and NSUN5 mRNA and protein expression levels in 120 patients with HCC (present study cohorts) were assessed using reverse transcription-quantitative PCR, western blotting or immunohistochemistry. The association between NSUN5 mRNA and protein expression levels and the clinical characteristics (or prognosis) of patients with HCC was analyzed using the χ 2 or log-rank test. The functions of NSUN5 in HCC were evaluated using in vitro and in vivo experiments, and the mechanism by which NSUN5 affected the progression of HCC was assessed using bioinformatics analysis using LinkedOmics. NSUN5 was significantly upregulated and predicted poor prognosis in HCC according to data from both TCGA database and present study cohorts.NSUN5 significantly promoted HCC proliferation and migration in vitro and significantly induced HCC tumor growth in vivo. Bioinformatics analysis demonstrated that NSUN5 was positively correlated with genes associated with translation in HCC. It was hypothesized that overexpression of NSUN5 strengthened ribosome functions and global protein translation, which may promote the proliferation and migration of HCC. In conclusion, NSUN5 may promote the progression of HCC by enhancing translation, thus making it a potential target for HCC treatment.

Background Chronic atrophic gastritis (CAG) is a pathological stage in the Correa’s cascade, whereby Helicobacter pylori (H. pylori) infection is the primary cause. Cellular senescence is an inducing factor for cancer occurrence and cellular senescence is an obvious phenomenon in gastric mucosal tissues of H. pylori‐positive CAG patients. Methods In this review, we collated the information on cellular senescence and H. pylori‐positive CAG. Results At present, only a few studies have observed the effect of cellular senescence on precancerous lesions. In combination with the latest research, this review has collated the information on cellular senescence and H. pylori‐positive CAG from four aspects‐ telomere shortening, DNA methylation, increased reacive oxygen species (ROS) production, and failure of autophagy. Conclusion This is expected to be helpful for exploring the relevant mechanisms underlying inflammatory cancerous transformation and formulating appropriate treatment strategies.

Purpose The object of this article was to assess the efficacy and safety of acupuncture and moxibustion therapy for patients recovering after surgical resection of Colorectal Cancer (CRC).Methods We systematically searched eight databases from the establishment of the database to October 2021 for randomized clinical trials (RCTs). We used the Cochrane risk of bias (ROB) tool to assess the ROB and the RevMan 5.3 for data analysis. The certainty of outcomes was summarized using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE).Results Twenty-seven studies involving the recovery of GI function met the inclusion criteria. There was significant variability and a high or unknown ROB. For certain outcomes reflecting physiological recovery, in comparison to sham acupuncture or usual care, acupuncture had a clear therapeutic effect, as measured by the time to first flatus [MD=−13.87, 95% CI (−17.82, −9.93), I2 =94%, p<0.00001], time to first defecation [MD=−15.25, 95% CI (−19.76, −10.75), I2 =94%, p<0.00001] and time to first bowel sounds [MD=−8.56, 95% CI (10.9, −6.23) I2 =91%, p<0.00001). Five studies reported possible adverse events of acupuncture and moxibustion, while the other studies did not premeditate adverse events.Conclusion Acupuncture and moxibustion are appropriate adjunctive treatments for postoperative GI function and they improve other symptoms, such as postoperative pain, to a certain extent for patients with CRC. For other symptoms, the efficacy remains uncertain due to a high ROB among studies. Future research should focus on not only more rigorous, well-designed and larger RCTs but also on assessing the biological mechanisms.