† This paper is an extended version of our paper published in Kupssinskü, L.; Guimarães, T.; Freitas, R.; de Souza, E.; Rossa, P.; Ademir Marques, J.; Veronez, M.; Junior, L.G.; Mauad, F.; Cazarin, C. Prediction of chlorophyll-a and suspended solids through remote sensing and artificial neural networks.Abstract: Total Suspended Solids (TSS) and chlorophyll-a concentration are two critical parameters to monitor water quality. Since directly collecting samples for laboratory analysis can be expensive, this paper presents a methodology to estimate this information through remote sensing and Machine Learning (ML) techniques. TSS and chlorophyll-a are optically active components, therefore enabling measurement by remote sensing. Two study cases in distinct water bodies are performed, and those cases use different spatial resolution data from Sentinel-2 spectral images and unmanned aerial vehicles together with laboratory analysis data. In consonance with the methodology, supervised ML algorithms are trained to predict the concentration of TSS and chlorophyll-a. The predictions are evaluated separately in both study areas, where both TSS and chlorophyll-a models achieved R-squared values above 0.8.Sensors 2020, 20, 2125 2 of 18 bodies [3]. Moreover, the suspended solids may indicate erosive processes within the watershed and additional water pollution since it can carry or store pollutants [4].Generally, once the samples get to a laboratory, their concentrations are analyzed, and manual measurements are taken to extract these parameters. The involved processes are costly, time-consuming, and require skilled personnel [5]. Although there are accurate evaluations, they do not indicate the individual spatio-temporal variation of water quality [6].In this sense, applications involving remote sensing can overcome the drawbacks of conventional monitoring since they allow real-time, spatial, continuous, and long-term monitoring in large areas [7,8]. The presence of components alters the spectral characteristics of the pure water, called Optically Active Components (OAC). The main OACs in water bodies are chlorophyll, suspended solids, and organic material [4]. The possibility of monitoring non-accessible areas and composing time series of water quality from historical data of remote sensing [9] are also factors that make this tool essential and its use promising.Among the most common satellites in OAC remote sensing studies are Landsat 8 and Sentinel-2. Although Landsat is more widely used, the European Sentinel-2 satellite has promising characteristics: it has higher spatial resolution than Landsat 8 and also spectral bands in the region called the red edge, which is of great interest for water and vegetation studies.Remote sensing serves as a powerful technique for monitoring environmental and seasonal changes, and its ability to remotely monitor water resources has increased in recent decades because of the quality and availability of satellite imagery data [10]. Even so, the analysis of small water bodies may not be adequ...