Resumo -O objetivo deste trabalho foi avaliar o uso de marcadores bioquímicos e fisiológicos, na caracterização do estresse hídrico em calos de cultivares de cana-de-açúcar (Sacharum sp.), RB 72 454 (sensível) e SP 813250 (resistente), contrastantes quanto à resistência à seca em campo. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial de 2x5 [cultivar x doses de polietilenoglicol (PEG)], com dez tratamentos e três repetições. Os calos foram submetidos a concentrações de PEG correspondentes aos potenciais osmóticos de 0, -0,3, -0,6, -0,9 e -1,2 MPa, por 120 horas. A variação no conteúdo relativo de água e na umidade não foi significativamente diferente entre as cultivares. Entretanto, foi observada a tendência de aumento no vazamento de eletrólitos, em conseqüência da diminuição do potencial osmótico na cultivar tolerante. Na cultivar sensível, observou-se tendência de aumento de prolina e, na resistente, diminuição, embora os níveis não tenham sido afetados pelo deficit hídrico. As concentrações de aminoácidos livres foram maiores na sensível. Houve queda nas concentrações de amônia, em ambas cultivares. Os níveis de proteínas não foram afetados pelo PEG. O perfil protéico por SDS-PAGE não mostrou aumento induzido por PEG, na intensidade das bandas correspondentes aos peptídeos entre 14 e 66 kDa. Os marcadores bioquímicos e fisiológicos não foram relacionados ao grau diferencial de resistência observado nas cultivares em condições de campo.Termos para indexação: Saccharum sp., ajustamento osmótico, cultivo de calos, solutos orgânicos. Alterations on the profile of nitrogenous fractions in sugarcane calluses induced by water deficitAbstract -The aim of this work was to evaluate the use of physiological and biochemical parameters, in the characterization of water stress in sugarcane (Sacharum sp.) calluses, cultivars RB 72454 (sensible) and SP 813250 (resistant), differing in drought resistance in the field. The experimental design was completely randomized with a factorial scheme 2x5 [cultivar x concentrations of polyethylene glycol (PEG)], with ten treatments and three replicates. Calluses were submitted to PEG concentrations corresponding to osmotic potentials of 0, -0.3, -0.6, -0.9 and -1.2 MPa, during 120 hours. The variation of relative water content and humidity showed no significant differences between the cultivars. However, it was observed a trend of increase in electrolyte leakage, in the resistant cultivar related to diminishig of the osmotic potential in resistant cultivar. In the sensible cultivar, it was observed a trend of increase in proline, while the resistant one showed decrease, though proline levels were not affected by the water deficit. The amino acid concentrations were bigger in the sensible cultivar. There was a decrease of ammonia in both cultivars. Protein levels were not affected by the PEG. The protein profile evaluated by SDS-PAGE showed no PEG-induced increase in the intensity of bands corresponding to peptides between 14 and 66 kDa. The physiological...
Neste trabalho foi avaliado o cultivo in vitro de somaclones de abacaxizeiro, na presença de elevadas concentrações de NaCl. Propágulos de abacaxizeiro foram submetidos a um período de cultivo in vitro em diferentes concentrações de NaCl (0, 12,5, 25 e 50 mM). Em seguida, foram cultivados na ausência do sal e, por fim, foram submetidos a um outro período de cultivo in vitro em 0 e 100 mM de NaCl. Não se observou interação entre as concentrações empregadas nos dois períodos de exposição ao NaCl, de modo que propágulos provenientes de tratamentos com concentrações mais elevadas não apresentaram melhor desempenho durante o segundo período de exposição; no entanto, constatou-se alteração negativa para os valores referentes à maioria das variáveis avaliadas em propágulos cultivados na presença de 100 mM de NaCl, na segunda exposição. Ao final do experimento foram selecionadas famílias cultivadas na presença de 100 mM de NaCl, com base na semelhança fenotípica ao controle. As famílias selecionadas eram originárias, igualmente, das quatro concentrações do primeiro período de tratamento com NaCl. Desse modo, as concentrações empregadas no primeiro período de exposição ao NaCl não influenciaram significativamente o crescimento dos propágulos durante o segundo período de cultivo in vitro na presença de NaCl.
This study focused on the use of herbal medicine in the municipality of Tavares (PB). The objective was to verify the use of phytotherapeutic treatment by users of basic health care in the municipality of Tavares (PB) and in compliance with recommendations of the Brazilian Pharmacopoeia. The work had the Basic Health Units – UBSs as locus. 100 semistructured interviews were conducted among users of the Basic Health Units between October and December 2016. Data were submitted to a non-parametric statistical analysis, at a significance level of 95%. As a result, 49% stated that they had chronic diseases, and arterial hypertension (44.07%) was the most cited one. The majority (62.50%) did not define medicinal plants and / or herbal medicines, but the majority (86%) responded to make use of them. Those who claimed to use medicinal plants cited 71 names and, in average, each respondent cited 4.49 (± 2.94). The most cited name was “cidreira” (13.83%). 42.71% reported having known the use of herbal medicines by mothers. There was no significant difference in the use of medicinal plants as a function of the municipal area (χ2 = 0,442, p = 0,506), of sex χ2 = 0,169; P = 0.506) and the health condition (χ2 = 2.719, p = 0.099). Regarding the number of names of plants with herbal properties mentioned, there was no significant difference as a function of the municipal area (U = 679.5, p = 0.2). However, there was a significant difference in relation to sex (U = 786.0, p = 0.033) and health status (U = 939.5, p = 0.031). Furthermore, a positive correlation (rss = 0.308; p = 0.002) was obtained between age and number of plant names cited. It is concluded that the users of basic health care in the municipality of Tavares (PB) use medicinal plants and the knowledge of their properties has a maternal origin. Female, older and self-reported chronic patients tended to cite a greater number of names of medicinal plants. More therapeutic actions of the mentioned medicinal plants were mentioned than the recommendations.
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