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Foundation is also greatly appreciated. I would also like to extend my gratitude to all the members of the Center for Advanced Technology and Education for creating an amazing research environment. In particular, I would like to acknowledge Zhenzhong Wang and Harold Martin, with whom I had the pleasure to collaborate with. vi

In order to meet the world food demand, agricultural biotechnology techniques are oriented at the productivity of crops in an efficient and sustainable manner. By linking society's demands with academia's resources, this document aims to evaluate the skills acquired by students in the field of microbiology during the generation of basic and introductory knowledge of microorganisms. The methodology included: Application of biotechnology techniques and Disciplinary Learning Outcomes evaluation. Results from the biotechnological process highlighted the characterization of the three types of funguses, which are more commonly found in soils: Trichoderma spp, Aspergillus spp, Fusarium spp. The Disciplinary Learning Outcomes evaluation showed that students reached mainly the developing and developed levels and a minimal part stayed at the level of excellence, since the first phase of the process was mechanical with respect to the second phase where the students must name or distinguish the structures under a microscope by a specialized website. The biotechnological techniques acquired at the university promote among future professionals a new perspective on clean production and the Disciplinary Learning Outcomes evaluation demonstrated their usefulness both in the detection of weaknesses and as an input for the curricular revision. Abstract-In order to meet the world food demand, agricultural biotechnology techniques are oriented at the productivity of crops in an efficient and sustainable manner. By linking society's demands with academia's resources, this document aims to evaluate the skills acquired by students in the field of microbiology during the generation of basic and introductory knowledge of microorganisms. The methodology included: Application of biotechnology techniques and Disciplinary Learning Outcomes evaluation. Results from the biotechnological process highlighted the characterization of the three types of funguses, which are more commonly found in soils: Trichoderma spp, Aspergillus spp, Fusarium spp. The Disciplinary Learning Outcomes evaluation showed that students reached mainly the developing and developed levels and a minimal part stayed at the level of excellence, since the first phase of the process was mechanical with respect to the second phase where the students must name or distinguish the structures under a microscope by a specialized website. The biotechnological techniques acquired at the university promote among future professionals a new perspective on clean production and the Disciplinary Learning Outcomes evaluation demonstrated their usefulness both in the detection of weaknesses and as an input for the curricular revision.