Purpose:To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. Methods: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) -treated with topical 0.5% povidone-iodine; Group II (natamycin) -treated with topical 5% natamycin; and Group III (control) -treated with topical saline solution. In all groups the rabbits were treated for three days and then sacrificed. The corneas were excised, macerated and immersed in 10 mL BHI. Culture samples were plated daily on Sabouraud's agar for 7 days, and the number of colony-forming units (CFU) was counted. The rabbits were clinically evaluated during the treatment period. Results: The povidone-iodine and natamycin groups demonstrated better efficacy than the control group based on the number of rabbits with no colonies growing. However, there were no statistically significant differences between the three groups when the number of CFU was analyzed (p>0.05). Conclusion: Our study demonstrates important methodological considerations in the use of in vivo animal models for the testing of antifungal agents. Using this sample size and methodology of counting CFU, topical 0.5% povidone-iodine demonstrated no benefit in the treatment of experimental Fusarium solani when compared with topical 5% natamycin.ABSTRACT

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INTRODUÇÃOO diagnóstico de glaucoma, classicamente se tem baseado na observação do disco óptico com características glaucomatosas, associado a defeitos correspondentes de campo visual, geralmente com níveis estatisticamente alterados de pressão intraocular (1) . Contudo, nem sempre o diagnóstico é evidente, principalmente em suas fases precoces. Desta forma, outras modalidades propedêuticas têm sido investigadas com a finalidade de proporcionar uma detecção mais precoce do dano glaucomatoso (1)(2)(3)(4)(5) . O exame da camada de fibras nervosas da retina (CFN) com o uso da polarimetria de varredura a laser foi introduzido na propedêutica do glaucoma, com o objetivo de proporcionar o diagnóstico precoce da doença através da medida da espessura da CFN (6)(7)(8) . Vários autores salientam que os defeitos na CFN precedem, em até anos, os observados no campo visual (9,10) . A perimetria com estímulo azul em fundo amarelo é realizada nos mesmos princípios que a técnica convencional com fundo e estímulo brancos e baseia-se nos relatos que as células ganglionares Análise de regressão logística na combinação de métodos propedêuticos no diagnóstico do glaucoma RESUMO