Fusarium oxysporum f.sp. cubense (Foc) is a soil-borne pathogen causing fusarium wilt banana disease. Management of soil-borne disease generally required the application of toxic pesticides or fungicides strongly affect the soil microbiomes ecosystem. Suppressive soil is a promising method for controlling soil-borne pathogens in which soil microbiomes may affect the suppressiveness. The comparative analysis of microbial diversity was conducted from suppressive and conducive soils by analyzing whole shotgun metagenomic DNA data. Two suppressive soil samples and two conducive soil samples were collected from a banana plantation in Sukabumi, West Java, Indonesia. Each soil sample was prepared by mixing the soil samples collected from three points sampling sites with 20 cm depth. Analysis of microbial abundance, diversity, co-occurrence network using Metagenome Analyzer 6 (MEGAN6) and functional analysis using Kyoto Encyclopedia of Genes and Genomes (KEGG) was performed. Data showed the abundance of Actinobacteria, Betaproteobacteria, Rhizobiales, Burkholderiales, Bradyrhizobiaceae, Methylobacteriaceae, Rhodopseudomonas palustri s, and Methylobacterium nodulans were higher in the suppressive than conducive soils. Interestingly, those bacteria groups are known functionally as members of Plant Growth Promoting Rhizobacteria (PGPR). The co-occurrence analysis showed Pseudomonas, Burkholderia , and Streptomyces were present in the suppressive soils, while Bacillus and more Streptomyces were found in the conducive soils. Furthermore, the relative abundance of Pseudomonas , Burkholderia , Bacillus , and Streptomyces was performed. The analysis showed that the relative abundance of Pseudomonas and Burkholderia was higher in the suppressive than conducive soils. Therefore, it assumed Pseudomonas and Burkholderia play a role in suppressing Foc based on co-occurrence and abundance analysis. Functional analysis of Pseudomonas and Burkholderia showed that the zinc/manganese transport system was higher in the suppressive than conducive soils. In contrast, the phosphate transport system was not found in conducive soils. Both functions are may be responsible for the synthesis of a siderophore and phosphate solubilization. In conclusion, this study provides information that PGPR may be contributing to Foc growth suppressing by releasing secondary metabolites.

<p><em>Abstrak</em> <strong>- </strong><strong><em>Blood Disease Bacterium </em></strong><strong>(BDB) adalah penyakit pada banyak tanaman<em> </em>bernilai ekonomis seperti tanaman pisang yang disebabkan oleh infeksi soil-borne bakteri patogen <em>Ralstonia solanacearum</em>. Kemampuan <em>R.solanacearum</em> untuk bertahan pada kondisi minimum pada tanah dan luasnya spektrum inang pertumbuhannya termasuk vektor perantara, menyebabkan belum ditemukannya strategi efektif untuk menanggulangi penyebaran penyakit BDB. Pengetahuan tentang respons tanaman pisang terhadap serangan <em>Ralstonia solanacearum</em>, merupakan langkah awal untuk memahami stategi tanaman dalam membentuk sistem pertahanan. Dalam penelitian ini, respons ekspresi molekuler gen-gen terkait pertahanan pada tanaman pisang kepok (<em>Musa paradisiaca</em>) dianalisis menggunakan teknik semikuantitatif real time PCR. Data pada penelitian ini menunjukkan <em>WRKY15</em> memiliki penurunan ekspresi pada hari ke-3 dan ke-7 pasca infeksi. <em>WRKY18</em> memiliki ekspresi paling rendah dibandingkan dengan <em>WRKY15 </em>dan<em> WRKY23 </em>serta tidak terdeteksi lagi ekspresinya pada hari ke-5 pasca<em> </em>infeksi karena <em>WRKY18</em> bersifat represi pada pengaturan sistem pertahanan tanaman basal. Level ekspresi <em>WRKY23</em> memiliki pola yang konsisten pada hari ke-1 sampai hari ke-7 pasca infeksi karena <em>WRKY23</em> merupakan salah satu early induced gene. Penelitian ini juga didukung dengan data morfologi yang menunjukkan gejala penyakit layu bakteri berupa penguningan daun pada hari ke-5 pasca infeksi dan pada hari-4 pasca infeksi telah terlihat koloni R.solanacearum pada pseudostem.</strong></p><p> </p><p><strong><em>Kata Kunci </em></strong><em>-<strong> </strong></em><em>Transkriptomik, WRKY, Ralstonia solanacearum, Penyakit Layu<strong> </strong>Bakteri</em></p><p> </p><p><em>Abstract - <strong>Blood Disease Bacterium</strong></em><strong> (BDB) is a disease in many economically valuable plants such as banana plants caused by soil-borne infection of pathogenic bacteria <em>Ralstonia solanacearum</em>. The ability of <em>R. solanacearum</em> to withstand minimum conditions on the ground and the extent of its host growth spectrum including intermediate vectors, has resulted in the lack of effective strategies to combat the spread of BDB. Knowledge about banana plant response to <em>Ranstonia solanacearum</em> attack, is the first step to understand crop strategy in forming defense system. In this study, the response of molecular expression of defense-related genes in banana kepok plant (<em>Musa paradisiaca</em>) was analyzed using semi-quantitative real time PCR technique. Data in this study showed WRKY15 had decreased expression on. WRKY18 has the lowest expression compared to WRKY15 and WRKY23 and no longer detected its expression on the 5th post-infection day because WRKY18 is repressive on the regulation of basal plant defense system. The expression level of WRKY23 has consistent pattern on the 1st day until the 7th post-infection day because WRKY23 is one of the early induced gene. This study was also supported by morphological data showing symptom of bacterial wilt disease in the form of yellowing of leaves on the 5th day post infection and on day 4 post infection has seen colonies R. solanacearum on pseudostem.</strong></p><p><strong> </strong></p><strong><em>Keywords</em></strong><em> - Transcriptomic, WRKY, Ralstonia solanacearum, Blood Disease Bacterium</em>