The prevalence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) as a cause of infectious disease in companion animals remains unknown. The emergence of MRSP is a challenge in veterinary medicine as multidrug-resistant strains began to emerge, resulting in treatment failures. This study provides an overview of the characterization of S. pseudintermedius strains from clinical pet samples and the prevalence of MRSP strains. A total of 123 S. pseudintermedius strains were characterized by phenotypic testing and the MALDI-TOF technique and evaluated for susceptibility to methicillin and the presence of the mecA gene. Of these, 49 (39.8%) were identified as MRSP. The results confirm the importance of monitoring resistant pathogens and the need for further studies to determine the prevalence of MRSP in companion animals. The prevalence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) as a cause of infectious disease in companion animals remains unknown. This study provides an overview of the characterization of S. pseudintermedius strains from clinical pet samples and the prevalence of MRSP strains. A total of 123 S. pseudintermedius strains were characterized by phenotypic testing and the MALDI-TOF technique and evaluated for susceptibility to methicillin and the presence of the mecA gene. Of these, 49 (39.8%) were identified as MRSP. The results confirm the importance of monitoring resistant pathogens and the need for further studies to determine the prevalence of MRSP in companion animals.

Acinetobacter spp. is emerging as an important human and veterinary pathogen, mostly due to intrinsic and acquired resistance to antimicrobials. Despite its public health relevance, little is known about the prevalence, role of different Acinetobacter species and antimicrobial resistance profile of animal-origin isolates. Traditional phenotypic tests may fail to discriminate Acinetobacter species, therefore molecular analyses are often required as a complementary approach. The objectives of this study were to evaluate the occurrence of strains of the Acinetobacter calcoaceticus-Acinetobacter baumannii (Acb) complex isolated from animal infections including urinary tract infections, otitis, piodermitis and pododermatitis, and its resistance profile against different antimicrobial classes, including carbapenems. All Gram-negative coccobacilli isolates were characterized by MALDI-TOF and multiplex PCR, and the disk diffusion test was used to investigate multi-drug resistance (MDR) and carbapenem resistance genes by PCR as preconized by the standard guidelines. MALDI-TOF technique identified 21 strains belonging to the Acb complex (10 A. pittii, 8 A. baumannii, 3 A. nosocomialis, 1 A. ursingii, and 1 A. venetianus). Multiplex PCR confirmed the results of MALDI-TOF for 20 strains. Eight strains (34.78%) were classified as MDR, being 50% (4/8) A. baumannii, 37.5% (3/8) A. pittii, and 12.5% (1/8) A. nosocomialis. None of the isolates presented phenotypic carbapenemase production. Considering the carbapenem resistance genes, 26.09% (6/23) of the isolates presented one or more carbapenemase genes. From these, 50% (3/6) presented only bla VIM, 33.33% (2/6) presented only blaIMP, and 16.67% (1/6) presented blaIMP e blaVIM, simultaneously. These genes were detected among A. pittii isolates mostly (66.67%, 4/6). This study provides further insights into the occurrence and resistance profile of Acinetobacter of animal origin.