In the research, the duck farm cushion and organic waste of duck hatching plant and slaughter and processing plant were used as compost materials, single factor 4 levels 3 replications design was carried out. Effects of adding different doses (0%, 0.35%, 0.40%0.40%, 0.45%) of microorganism agent (consisted ofthermophilic BacillusandSerratia) on compost physicochemical indexes including temperature, moisture content, C/N, germination index, total N, total P, total K, available N, available P, available K, and the enzymic activities including urease, cellulase, dehydrogenase, catalase and protease. There were four treatments in this experiment, three was with adding different doses (0.35%, 0.40%, 0.40%, 0.45%) of microorganism agents, one was without adding microorganism agent (control). The results showed that, adding microorganism agent treatment had faster temperature rising, higher temperature and longer high temperature duration than control. At the end of composting, C/N of those with adding microorganism agent were significantly lower than control (P<0.05), germination index, total N, total P, total K, available N, available P, available K were significantly higher than control (P<0.05). Urease, dehydrogenase, Catalase and protease activities were significantly higher than control (P<0.05), however, those with adding microorganism agent were not significantly higher than control (P>0.05).Enzyme activity of experimental treatments and the physicochemical index shows a good correlation. In conclusion, adding 0.35% microorganism agent was optimum for composting in this research.