Screening of High-performance Flocculant-producing Bacteria and Optimization of the Conditions for  Flocculation of Wheat Distillery Wastewater

Diao, Huan; Li, Lv Mu; Liang, Jun; Ding, Xinghong

doi:10.15376/biores.13.4.7738-7757

Cited by 5 publications

(3 citation statements)

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“…The bioflocculant from B. subtilis CSM5 demonstrated maximum flocculating activity when the bacterium was incubated at 30 • C. This might be due to the fact that the metabolic reactions of B. subtilis CSM5 were enhanced for its growth and bioflocculant production [40]. A rise in temperature above the optimum (>30 • C) might have tempered with the metabolic functions of B. subtilis CSM5, as denaturation might have set-in, consequently resulting in poor growth and bioflocculant production.…”

Section: Discussionmentioning

confidence: 99%

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Maliehe

Moganedi

Masoko

et al. 2022

Microbiology Research

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Bioflocculation has become the method of choice in wastewater treatment because of its effectiveness, environmental friendliness and innocuousness to humans. In this study, the bioflocculant-producing bacterium was isolated and its bioflocculant was used in wastewater treatment. The isolate was identified by 16S rRNA gene sequencing analysis. Its culture conditions (inoculum size, carbon and nitrogen sources, pH, temperature and time) were optimised using the one-factor-at-a-time assay. The cytotoxicity of the bioflocculant was assessed on human colorectal adenocarcinoma cells (Caco2) by tetrazolium-based colorimetric method. The ability of the bioflocculant to reduce biochemical oxygen demand (BOD) and chemical oxygen demand (COD) in wastewater was evaluated using Jar test. The bacterium was identified as Bacillus subtilis CSM5 and the maximum flocculating activity of 92% was observed when fructose and urea were used as nutrients and the culture conditions were adjusted to 30 °C, pH 9, 160 rpm and 72 h of incubation. Caco2 exhibited 90% viability when the highest bioflocculant concentration of 200 µg/µL was used. The reduction of BOD and COD was achieved at 59 ± 3.1 and 75 ± 0.4%, respectively. In conclusion, B. subtilis CSM5 is a good candidate for bioflocculant production and its bioflocculant has good potential for use in wastewater treatment.

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Section: Discussionmentioning

confidence: 99%

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Maliehe

Moganedi

Masoko

et al. 2022

Microbiology Research

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“…Microbial flocculants are produced at specific stages of microbial growth. The flocculating activity is determined by specific bioactivators, such as proteins, polysaccharides, and glycoproteins (Diao et al 2018(Diao et al , 2019. Microbial flocculants have been widely used in various industrial fields, such as wastewater treatment, microalgae harvest, activated sludge dewatering, heavy metal ion adsorption, and nanoparticle synthesis (Liu et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…However, there are few reports on the regulation of flocculation function genes on metabolic pathways. Therefore, a flocculant Klebsiella pneumoniae M1 strain (Preservation Number CCTCC M 2018098, GenBank login number MG987011) with strong flocculating function was screened from sediment sludge in this study (Diao et al 2018). The flocculant produced by the fermentation of this bacterium is mainly composed of polysaccharide and protein, with the contents of 65.9% and 19.74% respectively (Diao et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics analysis of Klebsiella pneumoniae M1, from microbial flocculation resources

Diao

et al. 2022

BioRes

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The synthesis of a microbial flocculant is strictly controlled by its genetic genes, which may be the result of the expression of flocculant genes in the microbial genome. The whole genome of Klebsiella pneumoniae M1 was sequenced for obtaining the mechanism of flocculant synthesis and exploring the mechanism of flocculant production by flocculant bacteria. Therefore, it provided a basis for molecular genetics and functional genomic analysis of flocculants production bacteria. In this way, the mechanism of flocculant production by flocculating bacteria can be better explored. The whole genome sequence of flocculant strain M1 was determined using advanced second generation (Illumina) and third generation (PacBio) sequencing, which was screened from wheat alcohol wastewater. The genes related to flocculant characteristics produced by strain M1 were analyzed for combining with the analysis of flocculant structure characteristics. According to the de novo assembly, a total of 5,511,794 bp clean reads were generated and assembled into 24 contigs. The GC content was up to 58.39%. The genome contained approximately 5383 genes, but 5348 genes had obvious biological functions. A total of 437 genes were involved in carbohydrate metabolism and had coding genes of five carbohydrate-related enzymes. This result indicated that there were functional genes closely related to polysaccharide production in M1 genome. The main metabolic process of flocculant strain Klebsiella pneumoniae M1 was closely related to the potential pathway of extracellular polysaccharide biosynthesis, in which five kinds of carbohydrate synthase genes were involved.

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Coagulant aids showed negligible effect on the flocculation capacity of the fermentation broth produced by a newly isolated strain of Serratia marcescens, XN-6

Duan

Cheng

et al. 2023

Environmental Technology & Innovation

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Screening of High-performance Flocculant-producing Bacteria and Optimization of the Conditions for Flocculation of Wheat Distillery Wastewater

Cited by 5 publications

References 0 publications

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Isolation of a Marine Bacterium and Application of Its Bioflocculant in Wastewater Treatment

Bioinformatics analysis of Klebsiella pneumoniae M1, from microbial flocculation resources

Coagulant aids showed negligible effect on the flocculation capacity of the fermentation broth produced by a newly isolated strain of Serratia marcescens, XN-6

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