The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

This study compared the effects of chicken dropping, NPK fertilizer, and Jatropha seed cake on the growth of bush okro (Corchorus olitorious L.), as well as its proximate and mineral composition. In a completely randomized design with three fertilizer treatments at four levels, soil samples were homogenized with 1, 2, 4, and 8 g/bag each of Jatropha seed cake, chicken dropping, and NPK fertilizer for the growth of bush okra. Standard techniques were used to assess the compositions of the proximate and minerals. The highest percentages of ash, lipid, fiber, nitrogen, crude protein, and moisture observed in Jatropha seed cake were: 16.5% (4g/bag), 3.5% (8g/bag), 11.5% (4g/bag), 1.15% (1g/bag), 7.18% (1g/bag), and 7% (8g/bag), NPK fertilizer: 21.5% (1g/bag), 3.0% (2g/bag), 10.5% (1g/bag), 1.27% (1g/bag), 7.96% (1g/bag), and 7.5% (2g/bag), Chicken dropping: 20.5% (8g/bag), 3.5% (4g/bag), 10% (2g/bag), 1.12% (8g/bag), 7% (8g/bag), and 6.5% (2 and 8g/bag) respectively.. All of the minerals that were examined (Na, K, Ca, and Mg) were found to be present in modest amounts. The composition of Corchorus olitorious L. proximate and mineral components were not significantly (p>0.05) affected by fertilizer types. The study's findings indicate that the application of Jatropha seed cake, which is comparable to NPK fertilizer and chicken droppings at 8 g/bag, accelerates the growth of C. olitorious. The findings support the use of Jatropha seed cake as a source of organic fertilizer, which may aid in the present need for food that is produced.

In vitro anti-malarial activity of petroleum ether extracts of Ficus sycomorus leaves was investigated using Candle Jar method. The extractions were carried out through activity guided fractionation. Petroleum ether extract has the highest activity, at the concentration of 1mg and 0.5mg with percentage growth inhibition of (33.12 and 31.9%) respectively. These results suggest that the leaves of Ficus sycomorus plant have significant antimalarial activity and that the antiplasmodial agents might be as a result of the extract fractionated using the petroleum.

The aim of this study was to investigate the antibacterial effect and identify the phytochemical constituents of Moringa oleifera leaves and seeds extract on Staphylococcus aureus (S. aureus) clinical isolates using agar well diffusion method. The samples were collected from the premises of Usmanu Danfodiyo University, Sokoto. The Seeds and Leaves were collected fresh. They were extracted using methanol and ethyl acetate. Various concentrations from 100mg/ml to 500mg/ml were prepared. The test bacteria used is Staphylococcus aureus obtained from Microbiology laboratory of the Usmanu Danfodiyo University. The bacteria were re-identified using biochemical tests. The bacterial inoculums were standardized to McFarland scale 0.5. Zone of inhibition were read after 24 hours of incubation at 370C.The results of the antibacterial study revealed that the methanolic leaves extracts at 500 mg/ml had effect on S. aureus with zone of inhibition of 20mm. The methanolic seed extract have effect on S. aureus with zone of inhibition of 19.5mm. The MIC for the leave and seed extracts for Staphylococcus aureus was 250mg/ml. The MBC was 500mg/ml. The results of the phytochemical analysis revealed the presence of flavonoid, tannins, saponins, cardiac glycosides, alkaloids, volatile oil, saponin glycosides, and glycosides but anthraquinone and steroids were absent in the extracts. The zones of inhibition showed that both the methanolic and ethyl acetate extracts at 500mg/ml were active to all the tested bacteria. ANOVA and Duncan Multiple Mean Range test was used to analyze the data. Based on Duncan’s grouping, there is significant difference between the solvents and the concentrations used.