The isoform composition of the 14.4 kDa profilin polypeptide was analyzed in seeds, leaves, flowers, roots and root-nodules from Phaseolus vulgaris L. Isoforms of pIs approximately 4.4-5 were present in all the tissues analyzed. The biochemical features of the protein present in seed tissue were determined. Seed profilin bound to Phenyl-Sepharose under low salt conditions which suggested a hydrophobic interaction; however, it was not associated with microsomal membranes nor it partitioned as a hydrophobic protein in Triton X-114. Fractions eluting from poly-L-proline or Phenyl-Sepharose columns contained well detectable amounts of profilin but no actin, suggesting that most of the protein was not present as profilactin in the seed. However, seed profilin appeared to be in some kind of complex since several molecular weight species were observed on native gels. In addition, profilin was found preferentially in the embryo axis and light microscopic immunolocalization showed a cytoplasmic distribution in this tissue.

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§ 3. Relation of the Stomata to Gaseous Exchange. § 4. Distribution and Dimensions of Stomata. § 5. Diffusion through the Stomata. § 6. Stomatal Movements and their Mechanism. § 7. Gaseous Exchange of Aquatics. § 8. Gaseous Exchange of Succulents. § 9, Energy Relations of Assimilation. § 10. Orientation of the Leaf and Illumination. § 11. Mechanism of Leaf Adjustment. § 12. Orientation of Strongly Insolated Leaves. § 13. Leaf Structure and Assimilation. § 14. Chlorophyll and Absorption of Light. § 15. Chlorophyll in its Relation to Assimilation. § 16. E.xternal Conditions and Assimilation. § 17. Assimilation in Natural Environment. § 18. Extent of Transpiration. § 19. Water Balance, Wilting, and Water Storage. § 20. Atmospheric Conditions and

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