Purpose The purpose of this paper is to know the effect of mediation of work motivation on the influence of job design and organizational culture on human resource performance. Design/methodology/approach The design of the study is the detailed planning used as a study guide that leads to the purpose of the study. The unit of analysis of this research is the expert and skilled labor of construction service actors working in the national consulting company PT. Yodya Karya (Persero) at the central level and branches spread over 11 branches in Indonesian territory. Data analysis method used is path analysis and Sobel test to test the indirect effect (mediation effect). Findings Based on the results of the analysis, several things can be concluded such as: there is a significant direct influence of work design on the performance of human resources and work motivation; there is a significant direct influence of organizational culture on work motivation and human resource performance; and there is a significant direct influence of work motivation on human resource performance. There is an indirect influence of work design on the performance of human resources through work motivation mediation variables. Meanwhile, work motivation is not the influence of organizational culture on human resource performance. Originality/value The originality of this research lies in forming the mediator variable that is the work motivation on the causal relationship of two variables and adding new variables of work design on the effect on the human resource performance.