No abstract

Successful reproduction of the plants is assumed as a crucial challenge for meeting nutritional needs of growing human population. One of the signi cant changes in the plant's life cycle is its transition from vegetative to reproductive phase. Obviously, the owering and bolting processes are in uenced by many genetic and physiological factors. In this way, several main regulatory pathways and effective genes have been identi ed, so far. The age-dependent phenomenon as an effective pathway of reproductive transition phase is in uenced by the miR172 and miR156 genes. In the present study, the miR156 sequence was identi ed in the sugar beet genome. First, the miR156 gene was cloned into the overexpression construct from the Beta vulgaris genome and then, was transferred to the beet explants. After the con rmation of transgenic plants, the transcript level of miR156 gene, and its target genes (SPL4 and SPL9) were evaluated in transgenic plants related to the control plants. Also, transgenic plants were studied in terms of ower phenotypic development, and root growth characteristics. By evidence evaluation, the research revealed that over-expression of miR156 played an effective role in reduction of SPL4 and SPL9 genes' expression that resulted in owering suppression in the Sea beet. According to the ndings, it had also a suppression effect on the root growth in transgenic beets.