Summary: By perfusion of the isolated human liver with collagenase and hyaluronidase a mixed suspension of various cell types was obtained. Pure parenchymal cells were prepared by differential centrifugation, pure non-parenchymal cells by the use of pronase and subsequent isopycnic centrifugation on metrizamide gradients (50-300 g/1). About 90% of the parenchymal and non-parenchymal cells were viable as judged by tiypan blue staining. Non-parenchymal cells were not capable of gluconeogenesis but utilized glucose at high rates. Parenchymal cells retained their ability to form glucose and to accumulate glycogen from fructose > lactate/pyruvate > alanine. Studies on binding of 125 Ilabelled insulin by isolated parenchymal cells were performed at 30 °C. The binding data may fit a model with a minimum of two classes of binding sites: (a) high affinity -low capacity sites (Kd ~~ 6.6 nmol/1, capacity ~ 16000 insulin molecules per cell) and (b) low affinity -high capacity sites (K d ~ 0.37 / , capacity ~ 646 000 molecules per cell).

This paper describes the very rare occurrence of subacute sclerosing panencephalitis (SSPE) in two siblings: a Turkish boy and his younger sister. The clinical picture was characteristic, and the diagnosis was confirmed in both cases by appropriate laboratory examination. The interval between the occurrence of the first neurological symptoms in the boy, and subsequently in the girl was four years. Study of HLA- and 27 other polymorphic marker-systems did not reveal linkage to one of the systems tested. Therapeutic trials in the girl included intravenous and intraventricular application of a total of 87 X 10(6) U human fibroblast interferon (Hu INF-beta) over 21 days. However, up to 3 months after the end of interferon administration there were no significant changes in the girl's condition.

Eine enzymatische Methode zur Isolierung intakter Parenchymzellen aus Rattenlebern mit einer Zellausbeute von 3-4 g Feuchtgewicht aus etwa 10 g schweren Lebern von 180-230 g schweren Tieren wird beschrieben. Nach einer in-vitro-Vorperfusion mit einem calciumfreien Medium wurde Kollagenase (200 mg/1) und Calciumchlorid (5,2 mmol/1) hinzugegeben und die Leber 15 Minuten bei 37°C perfundiert. Die mikromorphologische Unversehrtheit der Zelloberfl che wurde mit dem Rasterelektronenmikroskop nachgewiesen. Mit dieser Methode isolierte Leberparenchymzellen bertrafen hinsichtlich der Gluconeogenese-und Proteinsyntheseraten Leberschnitte und nach anderen Verfahren gewonnene Zellen. ATP/ADP-und Lactat/Pyruvat-Quotienten als Parameter f r den Energie-bzw. Redoxstatus lagen mit 5,69 bzw. 8,64 im physiologischen Bereich. Die Integrit t der membranst ndigen Hormonrezeptoren wurde durch den Nachweis der Sensibilit t gegen ber Adrenalin, Glukagon und Insulin belegt. Die Glykogendeposition in Hepatocyten gefasteter Ratten wurde durch Glukagon (0,3 μτηοΐ/ΐ) und Adrenalin (l μιηοΐ/ΐ) um 84,9 bzw. 95,9 % vermindert. hnlich wirkungsvoll stimulierten beide Hormone die Glykogenolyse in Parenchymzellen gef tterter Tiere. Die Harnstoffsynthese wurde durch Glukagon (l μπιοΐ/ΐ) um 29,5 % stimuliert und durch Insulin (10 nmol/1) um 28,5 % gehemmt. Insulin unterdr ckte in 10 nmolarer Konzentration den Glukagoneffekt auf die Harnstoffbildung. Isolation of intact liver parenchymal cells by a modified enzymatic metliodAn enzymatic method is described for isolating intact parenchymal cells from rat livers. 3-4 g cells (wet weight) could be isolated from livers of rats weighing 180-230 g. After an in vitro preperfusion of 15 minutes with a Ca-free buffer, collagenase (200 mg/1) and calcium chloride (5.2 mmol/1) were added. Perfusion was continued for another 15 minutes at 37°C. Micromorphological integrity of cell membranes was demonstrated by scanning electron microscopy. With regard to rates of gluconeogenesis and protein synthesis, parenchymal cells isolated according to our method were found to be superior to liver slices and cells isolated by other methods. Ratios of ATP/ADP (5.69) and of lactate/pyruvate (8.64) as parameters of the energetic situation and the redox state resp. were found within the physiological range. Integrity of cell surface receptors was proved by their sensitivity to epinephrine, glucagon and insulin. Glucagon (0.3 μιηοΐ/ΐ) and-epinephrine (1 μηιοΐ/ΐ) reduced glycogen deposition in hepatocytes of fasted rats by 84.9 % and 95.9 % resp. Both hormones stimulated glycogenolysis in parenchymal cells of fed rats to a similar extent. Urea synthesis was stimulated 29.5 % by glucagon (1 μιηοΐ/l), and inhibited 28.0 % by insulin (10 nmol/1). The stimulatory effect of glucagon (1 //mol/1) was abolished by insulin (10 nmol/1):

DNA of unrelated persons as well as members of families that were totally or partially homozygous or completely heterozygous on the loci of the major histocompatibility class I genes has been isolated from peripheral blood lymphocytes and blot hybridized with the class I pseudogene pHLA 12.4 probe. The autoradiographic DNA patterns were discussed and compared with well-defined serological features. Positive associations with serologically typed alleles had been demonstrated for HLA-A1,11; -A2; -A3; -B7; -B14; -B35; -Bw41; and -Cw5.

An apparently new EsD gene product (EsD*Düsseldorf) was detected by use of horizontal agarose gel electrophoresis (AGE), starch gel electrophoresis (SGE), and isoelectric focusing (IEF). The observed phenotype EsD (1-Düsseldorf) can be distinguished from any known EsD type.