alpha-Linolenic acid (ALA) is a major dietary (n-3) fatty acid. Some clinical trials with ALA supplementation have shown reduced cardiovascular risk; however the specific cardioprotective mechanism is not known. We studied the effects of daily supplementation with ALA derived from flaxseed oil on concentrations of plasma LDL cholesterol, HDL cholesterol, intermediate density lipoprotein cholesterol, and lipid particle sizes. In a randomized double-blind trial, 56 participants were given 3 g/d of ALA from flaxseed oil in capsules (n = 31) or olive oil containing placebo capsules (n = 25) for 26 wk. Changes in plasma HDL cholesterol, LDL cholesterol, and triglyceride concentrations did not differ between the 2 groups at 26 wk. The adjusted plasma total cholesterol concentration at 26 wk was 0.45 mmol/L higher in the flaxseed oil group (5.43 +/- 0.03 mmol/L) compared with the olive oil group (5.17 +/- 0.07 mmol/L) (P = 0.026). ALA did not affect LDL, HDL, or IDL particle size; however, the concentrations of the large, less atherogenic LDL1 (P = 0.058) and LDL2 (P = 0.083) subfractions tended to be greater in the ALA group. In conclusion, ALA does not decrease CVD risk by altering lipoprotein particle size or plasma lipoprotein concentrations.

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ContextSoft-release involving supplementary feeding or shelter is commonly used in wildlife reintroduction and rehabilitation projects. However, competition for nestboxes and supplementary feed, as well as predation at feed stations or nestboxes, can reduce the benefits of soft-release. The use of microchip-automated technology can potentially alleviate these concerns, by providing targeted supplementation to only the intended, microchipped animals. AimsWe aimed to train wild-caught northern brown bandicoots, Isoodon macrourus, to use microchip-automated doors to access safe refuge. MethodsBandicoots were trapped from the wild and brought to the Hidden Vale Wildlife Centre, where eight were trained to use the doors in a six-stage process, and then six were trained in a three-stage process, using a peanut butter reward. Key resultsBandicoots learned to use the doors in as few as 3 days. The duration of visits to the door generally increased during training, although the number of visits decreased. ConclusionsThe bandicoots successfully learned to use the microchip-automated doors, which shows that this technology has great potential with wildlife, particularly given the short training times required. ImplicationsThe use of these microchip-automated doors with wildlife has many potential applications, including supplementary feeding stations, nestboxes, monitoring in the wild, as well as enrichment for wild animals in captivity.