Several cases of consumer complaints to the still soft beverages – fruit drinks and ice teas were analysed. The visible impurities and slight sensory changes were observed, sediment was formed by microbial cells. The bacteria were isolated and identified as <I>Asaia</I> sp. The identification was confirmed by PCR technique. The sensitivity of bacteria to preservative agents especially to the sodium benzoate, potassium sorbate and dimethyldicarbonate was evaluated in model samples and in the real conditions of the beverage. The minimal inhibitory concentrations were estimated as well as the factors affecting the sensitivity of bacteria. Besides the stabilisation of drinks the general possibilities of decay prevention were considered, the efficiency of cleaning and sanitation procedures were evaluated, including the comparison of various sanitation agents.
Sedláčková P., Čeřovský M., Horsáková I., Voldřich M. (2011): Cell surface characteristic of Asaia bogorensis -spoilage microorganism of bottled water. Czech J. Food Sci., 29: 457-461.The ability of bacteria to attach to a surface and develop a biofilm has been of considerable interest for many groups in the food industry. Biofilms may serve as a chronic source of microbial contamination and the research into biofilms and cells interactions might help to improve general understanding of the biofilm resistance mechanisms. Multitude of factors, including surface conditioning, surface charge and roughness and hydrophobicity, are thought to be involved in the initial attachment. Hydrophobic interactions have been widely suggested as responsible for much of the adherence of cells to surfaces. Cell-surface hydrophobicity is an important factor in the adherence and subsequent proliferation of microorganisms on solid surfaces and at interfaces. In the present study, we have estimated the cell-surface characteristics of Asaia bogorensis -isolated contamination of flavoured bottled water and compared its ability to colonise surfaces which are typical in the beverage production -stainless steel, glass and plastic materials.
e inactivation e ect of the combination of nonthermal plasma and hydrogen peroxide aerosol for the microbial decontamination of inner surface of cylindrical container buckets is studied on one bacterial and seven lamentous micromycete species and on airborne-contaminated cups. While the decontamination by single nonthermal plasma or hydrogen peroxide is not observable after 120 s, the strong decontamination by their combination occurs after 30 s of exposure. Moreover, observed total elimination of airborne contamination of plastic cups predetermines this method as a suitable alternative to the currently used method based on the application of hydrogen peroxide.
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