No abstract

The rate of cell production in thirty‐five cases of carcinoma in Bilharzial bladder was evaluated from the labelling index after in vitro incubation with [3H]TdR. Squamous cell carcinoma was the most frequent histological type in this series and had a median LI of 8.0% which corresponds to a potential doubling time of 5.9 days. In squamous cell tumours the LI increased with the histological grade. Transitional cell tumours had a somewhat greater LI. In all histological types the LI was significantly greater in the deep infiltrating parts of the tumour than in the superficial parts. The discrepancy between the estimated potential doubling time and the growth rate normally attributed to such tumours suggests the existence of an extensive cell loss factor. Areas of focal or diffuse mucosal hyperplasia were associated with increased LI.

Background and Objective: Colorectal Carcinoma (CRC) is the most common malignancy of the gastrointestinal tract. The overall five-year survival for colon cancer and rectal cancer are 65% and 68%, respectively with liver metastasis being the leading cause of death. Despite the use of active targeted drugs for treatment of metastatic CRC in the past decade, cure rates remain low. Therefore, research on novel markers for earlier diagnosis and target therapy of CRC is of a great concern. OTUB 1 (OTU deubiquitinase, ubiquitin aldehyde binding 1) is a deubiquitinating enzyme (DUB) that belongs to the OTU (ovarian tumor) superfamily. The aim of this work is to study the immunohistochemical expression of OTUB 1 in CRC and its correlation with the available clinicopathological variables. Material and Methods: This retrospective study was conducted on a total number of 45 cases of CRC. All cases were retrieved from the archival files of the pathology labs of Ain Shams University Hospitals (from 2012-2016). Immunohistochemistry using rabbit polyclonal anti OTUB 1 antibody was performed. Results: OTUB 1 was overexpressed in CRC tissues, and the expression level of OTUB 1 was correlated significantly with histologic grade (p<0.001), depth of invasion (pathological T) (p=0.03) and location of CRC (p=0.002). On the other hand, no significant correlation was found with age, gender, metastasis whether nodal or distant, TNM stage and size of tumor. Conclusion: OTUB 1 may have a possible role in tumor differentiation as well as local tumor invasion, thus could be useful in identification of biologically aggressive tumors in colonoscopic biopsies in addition to providing a basis for targeted therapy which could play a major role in the treatment of CRC.