Local muscle fatigue was investigated in the anterior temporal and masseter muscles during sustained isometric contractions. The volunteers' subjective perception of fatigue or pain was recorded at intervals during the sustained contractions, and this level was then compared to objective measures of fatigue. These objective measures included shift in median frequency of the power spectrum of the surface-detected EMG signal, and change in signal amplitude. The relationship was investigated while closing on a bite force meter placed between the second premolars and first molars unilaterally, and while simply clenching with the teeth together without the bite force meter. It was found that the subjective perception of fatigue, as measured on a visual analogue scale (VAS), had a nearly linear relationship with time, and that the relationship between the VAS score and median frequency shift was rather closer than the relationship with amplitude change.

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