In a situation of ambiguity and global crisis, uncertainty tolerance, purpose or meaning in life and life values have become important factors of psychological health and adaptability of the Transport University students. The purpose of this study is to survey the levels of students’ uncertainty tolerance, purpose in life and life values.Sixty students of Saint-Petersburg State Transport University aged 18–22 participated in the survey. The following methods were used in the research: Crumbaugh and Maholic’s PIL (Purpose-in-Life Test) adapted by D. Leontyev; V.F. Sopov and L.V. Karpushina’s Morphological Test of Life Values; E.N. Osin’s adaptation of the McLane Uncertainty Tolerance Scale.The Purpose-in-Life Test have revealed average scores; at the same time, high scores have prevailed on the “Locus of Control – Life” scale. The students’ material and spiritual values proved to be in a good balance. The students’ Uncertainty Tolerance turned out to be at an average level, but the “Uncertainty Tolerance” scale have revealed either high-level or low-level scores.Uncertainty tolerance is related to life values in both males and females. A higher purpose in life tends to promote greater psychological adaptability to ambiguous situations and boosts uncertainty tolerance in general. The females’ uncertainty tolerance is connected to spiritual values, maintaining individuality, creativity, and hobbies. The males’ attitude to novelty is linked to their prestige and achievement.According to the survey, the Transport University students have an average level of uncertainty tolerance, purpose in life, and well-balanced material and spiritual values. Purpose in life is related to higher uncertainty tolerance and adaptability to ambiguous situation. There is some gender specific correlation between life values and uncertainty tolerance among males and females. Females’ attitude is more related to spiritual values whereas males are more oriented to social success.