This study was conducted to evaluate the ability of some fungal culture filtrate, as biocontrol agents against okra wilt caused by Fusariumsolani. and Meloidogynejavanica. In the present study, fungal culture filtrates (FCFs) of Aspergillusterreus (1), Aspergillusterreus (2), Penicilliumchrysogenum, and Trichoderma spp. were tested against M.javanica in vitro. The effects of P.chrysogenum and Trichoderma spp. (FCFs) in controlling root-rot fungi and root-knot nematode disease complex on okra plants were studied under greenhouse conditions (In vivo). In vitro experiment, the results revealed cumulative rate of J2s mortality of M.javanica reached to 97.67 and 95% by P.chrysogenum and Trichoderma spp., respectively, after 72 h. incubation. Additionally, Trichoderma spp exhibited the most effective inhibitory activity against the pathogen's radial growth, with a percentage of 68%. P.chrysogenum ranked second with 53.88%, while A.terreus (2) demonstrated the weakest inhibitory effect of 24.11%. T6 [Nematode infection (M.javanica) + Fungus infection (F.solani) + Overflowed with fungal culture filtrate (P.chrysogenum)] and T8 [Nematode infection (M.javanica) + Fungus infection (F.solani) + spray with fungal culture filtrate (P.chrysogenum)] had the greatest effects on nematode galling indices on okra roots and substantially reduced the reproductive factors in the greenhouse (In vivo experiment). T6 was the best treatment to decrease disease severity, as reached (28%) relatively. On the other hand, T12 [(Fungus infection (F.solani) + (Dovex 50% fungicide with irrigation water)] recorded the lowest disease severity reaching (8%) relatively. The results showed that nematode infection or fungus infection or both decreased all studied anatomical characteristics of okra root, stem, and leaves. We concluded from this study that root-knot nematode and root-rot fungi were reduced by using fungal culture filtrates and could improve plant growth.

This study was conducted to evaluate the ability of some endophytic fungi, as biocontrol agents against sesame wilt caused by Fusarium oxysporum f. sp. sesame. Eleven fungal species belong to genus Fusarium was isolated from different localities in Sohag and Assiut governorates. All isolated species proved to be pathogenic on Giza-32 sesame cultivar. Twelve endophytic fungi isolates were isolated from some parts of various plants. The endophytic fungi isolates were tested for antagonistic activity by the dual culture and culture filtrate techniques. All endophytic fungal isolates were showed antagonistic activity against the causal pathogen in vitro. The endophytic Aspergillus niger was more antagonistic than other tested isolates toward the causal pathogen followed by A. clavatus, Penicillium chrysogenum and P. crustosum. All the tested fungal culture filtrates (FCF) were significantly affected the growth of the causal pathogen. FCF of Aspergillus terreus isolate at 20 % concentration displayed the greatest inhibitory action to the pathogen mycelia growth. Single application with endophytic fungi or combined with Trichoderma sp. were significantly deceased the disease severity (%) and increased the plant growth parameters such as shoot length, shoot weight, root weight and number of pods.

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