Abstract. We propose to detect the use of γ-rays in order to radiosterilize ampicillin by UV absorption spectroscopy. The UV absorption results are verified by comparison with HPLC-diode array analysis. The sensitivity is excellent (10 -6 M). The wavelengths in the range from 287 to 335 nm are characteristics of radiolysis related substances. It is possible to use this range of wavelengths to detect the radiolysis of ampicillin with an usual UV spectrophotometer.
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