This experiment was carried out in the laboratories of fish and animal resource centeragricultural research directorate from 1/3-30/6/2015 to study the possibility of improving the nutritional value of local grass pea seeds Lathyrus sativa (GPS) by degradation of anti-nutritional factor and using it as protein source for common carp Cyprinus carpio L. diets. Three different treatments for GPS meal, fermented, germinated and soaking (GPS) were used as partial or total replacement of soybean meal (SBM) for practical diets of common carp Cyprinus carpoi L. ration. Thirteen experimental diets were formulated, the diets 1, 2 and 3 were used crud GPS without any treatment at the substitute ratio 33%, 66% and 100% of (SBM), the diets 4, 5 and 6 contained fermented GPS at the same substitute ratio. The diets 7, 8 and 9 were germinated GPS at the same substitute ratio. The diets 10, 11 and 12 were soaking GPS at the same substitute ratio and diet 13 for control without GPS. The results showed no significant differences between control treatment (T 13) without GPS and soaking at substitution ratio 33% and 66% (T 10 and T 11), which were significantly differed (P<0.05) with other treatments. Thereby, it is recommended soaking GPS and at substitute 33% and 66% of SBM for common carp diets.
A total of 120 common carp Cyprinus carpio L. with average weight 20.45±1.24 gm/fish were assigned randomly into five groups of three replicates for each group, each replicate included 8 fishes. The fish were fed for 90 days on five diets that equal in isonitroenons 24.16% and isocaloric 1408.88 MJ/kg. Diet for control T1, local probiotic Iraq probiotic added at levels 5g/kg for diet T2, local probiotic added at 5g/kg from diet+5g/kg prebiotic which include fructo-Oligosacchardes FOS for diet T3, imported probiotic which include Enterococcus faecium added at 2g/kg diet for diet T4, imported probiotic which include E. faecium added at 2g/kg diet+2g/kg per diet prebiotic which include FOS for diet T5. The results showed there were significant distinction for T5 p<0.05 in growth parameter and food conversion and sufficiency rate FCR and FER and apparent coefficient digestibility ACD, there were no significant different between T5 and T4. The results of blood biochemical examination showed significant distinction for T4 from T1 and T2 in packed cell volume (PCV) and Hemoglobin Hb, there were no significant different between all treatment in erythrocyte and leukocyte count RBC and WBc.
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