Polyaromatic hydrocarbons (PAHs) are worldwide contaminants that can reach toxic concentrations that are detrimental to the environment and human health. In this study, three different isolates (Klebsiella oxytoca, Klebsiella pneumonia and Acinetobacter sp.) from crude petroleum oil samples were found to be PAHs utilizers. They are able to utilize four different PAHs, phenanthrene, fluoranthene, pyrene, and benzene. K. oxytoca is the most efficient utilizer of the PAHs rather than K. pneumonia and Acinetobacter sp. It can utilize almost 83% of benzene after 48 h of shaking, if supplemented with nitrogen source. The isolates have been molecularly identified by partial sequencing of the 16S rDNA gene (approximately 900 bp) and the results demonstrated a high degree of homology to K. oxytoca (up to 99% similarity), K. pneumonia (from 97 to 99% similarity) and Acinetobacter sp (up to 99% similarity).