Polyaromatic hydrocarbons (PAHs) are worldwide contaminants that can reach toxic concentrations that are detrimental to the environment and human health. In this study, three different isolates (Klebsiella oxytoca, Klebsiella pneumonia and Acinetobacter sp.) from crude petroleum oil samples were found to be PAHs utilizers. They are able to utilize four different PAHs, phenanthrene, fluoranthene, pyrene, and benzene. K. oxytoca is the most efficient utilizer of the PAHs rather than K. pneumonia and Acinetobacter sp. It can utilize almost 83% of benzene after 48 h of shaking, if supplemented with nitrogen source. The isolates have been molecularly identified by partial sequencing of the 16S rDNA gene (approximately 900 bp) and the results demonstrated a high degree of homology to K. oxytoca (up to 99% similarity), K. pneumonia (from 97 to 99% similarity) and Acinetobacter sp (up to 99% similarity).

In this study, 23 Salmonella isolates were detected by polymerase chain reaction (PCR) from human fecal samples obtained during 2010 from local hospitals and clinical laboratories in Kingdom of Saudi Arabia (KSA). The bacteria were cultured, serotyped and biochemically characterized by the analytical profiling index (API 20E). The invA and hilA gene primers were selected specifically for the detection of Salmonella to amplify a 284 and 845 bp DNA fragments, respectively. Only 3 isolates out of 23 were not identified or typed neither by API 20E nor serological tests. One of these isolates (S5) had 98% sequence similarity with the invA gene sequences of Salmonella typhimurium strain LT2, 14028S and SL1344. The results showed that invA or hilA PCRs specificity was 66.6% compared to API 20E and serology for Salmonella enterica identification in clinical specimens. The PCR assays compared to both biochemical and serological tests were able to specifically detect all of 23 Salmonella isolates (100% sensitivity).

Problem statement: In this study, the effects of Relativistic Restricted three-Body Problem (in brief RRTBP) on the equilibrium points of both triangular and collinear is considered. The approximate locations of the collinear and triangular points are determined. Series expansions are used to develope in µ and 1/c 2 as small parameters. To check the validity of our solution, when ignoring 1/c 2 terms we get directly the classical results of the restricted three-body problem. Conclusion/Recommendations: A MATHEMATICA program is constructed to give a numerical application of the relativistic perturbations in the locations of the equilibrium points of the three body problem.

Background: Cesarean section is the birth of a fetus through an incision in the abdominal wall and the uterine wall. It classified according to time of performing it into elective cesarean and emergency cesarean section. Aim: The aim of the study was to assess the quality of life among women undergoing elective cesarean section versus emergency cesarean section. Setting: This study was conducted in postpartum unit at Benha university hospital. DESIGN: A descriptive exploratory study design was utilized. Sampling: A Purposive sample included 200 women. Tools: Structured interviewing questionnaire sheet, Visual analogue pain scale and Quality of life questionnaire sheet. Results: The present study revealed that there was no significance difference in women's knowledge regarding cesarean section and quality of life between two groups. Pain increased in emergency group than elective group. There was highly statistically significance difference regarding quality of life (P ≤ 0.001). Women in elective group had better quality of life than women in emergency group in all domains. Conclusion: Women knowledge regarding cesarean section and quality of life was unsatisfactory. The pain score was severe in emergency than elective group. Elective cesarean section had better quality of life than emergency cesarean section. Recommendations: developing awareness program for enhancing woman knowledge for improving quality of life post cesarean section.