Aim. To investigate the microbiota of honeycombs with affected bee brood. Methods. Visual, immunochro- matographic, cultural-morphological, biochemical, electron-microscopic methods were used to isolate and previously identify a number of microorganisms. Results. 10 samples of honeycombs with sealed brood were studied. The following agents of bee diseases were isolated and previously identifi ed: Paenibacillus larvae – American foulbrood; Вasillus paraalvei – parafoulbrood; Melissococcus pluton – European foulbrood. Con- clusions. The number of cocci, spore-forming coli and yeasts, found in the samples of honeycombs, commonly represent normal microfl ora of bees, but their number increases signifi cantly in case of viral and bacterial infections.