No abstract

-Experiments on use of an agar-gel method for recovery of migrating Ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. Subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% NaCl at 38°C. The results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agargel and more than 83% of the obtained larvae migrated out of the lung agar-gel. The larvae were subsequently available in a very clean suspension which reduced the sample counting time. Blending the liver for 60 sec in a commercial blender showed significantly higher larvae recovery than blending for 30 sec. Addition of gentamycin to reduce bacterial growth during incubation, glucose to increase larval motility during migration or ice to increase sedimentation of migrated larvae did not influence larvae recovery significantly. Ascaris suum; larva recovery; agar-gel method; liver; lungs.

It is rare for solitary basal cell cancer not to be associated with the naevoid basal cell carcinoma syndrome (NBCCS), xeroderma pigmentosum, an organoid nevus or X-ray therapy in children (to date 86 cases have been documented in the literature. Aetiologically, the tumours might be a forme fruste of the NBCCS or they might follow a somatic point mutation of keratinocytes. Up to now, data on the repair mechanism following UV-induced DNA damage are not available in these patients. We report on a 10-year-old boy with a solitary nodular basal cell cancer in the right malar region. Neither the patient's history nor the clinical findings suggested a genetic disposition to development of the tumour.