In this research, the cultivation and storage of potatoes grown in saline soils of Khorezm region at different levels with biopreparations, such as Bist (Pseudomonas putida) and Zamin-M (Bacillus subtilis, Bacillus megaterium and Pseudomonas stutzeri), were studied. In the experimental variants, there was 1.381 mg/kg of vitamin C, followed by 0.112 mg/kg of vitamin B6, 0.089 mg/kg of vitamin PP, 0.481 mg/kg of vitamin B12 and 0.092 mg/kg of vitamin B2 when Zamin-M was applied. Potatoes, which were treated with Zamin-M, contained 0.45% Fructose, 0.87% Glucose, 0.92% Sucrose, 0.04% Maltose, and it was higher than other biopreparations. During storage, the number of diseased nodules was 21.8% when treated with Earth ointment, it was 20% when treated with Bist, and it was 19.8% when treated with Zamin-M. The results showed that Zamin-M was suitable in soil and climate conditions of Khorezm region, especially for storage of potato, and it was found that Zamin-M contained microorganism, which activate the synthesis of phytohormones operating under stress. In comparison, the tested Zamin-M extended the shelf life of potatoes by 25% than the control variants.
This paper investigates the cultural-morphological, physiological and biochemical properties of bacterial isolates with stimulating properties isolated from the rhizosphere of cotton (Gossýpium hirsútum) grown in saline soils of Uzbekistan. The cells of the isolated culture No. 12 were found to be rod-shaped, 2-3x0.5-0.6 urn in size, single or chain-linked, Gram-positive aerobic bacteria producing spores. In studying the culture No. 146, its cells were in the form of thick, less mobile rods, 1-2x0.6-0.8 urn in size, forming thermostable spores. The spores were located in the center of the cell; the Gram-stained colonies in meat-peptone agar were round, bulging; the edges were flat, consistency oily, smooth and, mucous; and, the upper part was found to be shiny. Studies have shown that the isolates appertained to the genus Bacillus sr.
From the turn of the 20th century, biopreparations that quicken plant growth and development have been frequently employed. The accumulated knowledge of the mechanisms governing interactions between microorganisms and plants necessitates the use of minimal resources and energy in the targeted design of phytomicrobial systems and the optimization of their adaptability in order to increase soil fertility and plant productivity, increase plant yield, and increase plant resistance to diseases and unfavorable environmental conditions and stress factors. In the article today, enough experience has been collected on the scientific management of soil microorganism processes in the optimization of agricultural production and maintaining soil fertility, and it envisages the creation and use of microbiological preparations as the main link of intensive technologies in plant science. It is known that concentration of biomass of microorganisms using flocculants in the technological processes of production of bacterial preparations, after the stage of growth in enzymes, the stage of concentration of biomass of microorganisms from culture liquid is carried out.
In this article, a microbial inoculant acting as a biofertilizer is considered as a biostimulant, consisting of three strains of local salt-tolerant strains of rhizobacteria Pseudomonas stutzeri SKB308, Bacillus subtilis SKB309, Bacillus megaterium SKB310. The authors have developed and introduced into agricultural practice an experimental sample of the biological product ‘Zamin-M’, which increases the resistance of cotton to stress conditions of soil salinity. It is proved that the biological product ‘Zamin-M’ increases soil fertility has a beneficial effect on the enzymatic activity of the soil and the development of a population of beneficial microorganisms. The bioproduct ‘Zamin-M’ is presented as a new stimulant in the practice of growing cotton in saline conditions. An agricultural technique for the use of a local biological product in agriculture has been developed. The ‘Zamin-M’ is included by the State Committee of the Republic of Uzbekistan in the list of agrochemicals and pesticides permitted for use in agriculture of the Republic of Uzbekistan (certificate No. 1A1005).
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