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The objective of this study was to evaluate the influence of energy supplementation on associative effects between forage and concentrate supplements and on the performance of beef cattle on a tropical pasture during the rainy season. Twenty-eight Nellore bulls averaging 19 months of age and 325.2 ± 4.1 kg were allotted to a completely randomized design and distributed into four paddocks of two hectares each with a tropical pasture for 84 d. The treatments consisted of bulls on Urochloa brizantha cv. Xaraés pasture without concentrate supplementation or bulls on Urochloa brizantha cv. Xaraés pasture receiving concentrate supplementation at 15 (S15), 30 (S30) or 45% (S45) of the total digestible nutrients (TDN) requirement All bulls had ad libitum access to mineral supplementation. The amount and chemical composition of the supplement were adjusted every 28 d. Forage and supplement intakes were evaluated in all animals using fecal markers. Crude protein: digestible organic matter ratio decreased with supplementation; consequently, the imbalance between dietary protein and energy increased. Forage intake decreased with increasing supplementation. The substitution rates of pasture for supplementary feed were 0.14, 0.33, and 0.46 for bulls from S15, S30, and S45 treatments, respectively. However, the apparent DM digestibility increased linearly with supplementation level. Supplementation improved the assimilation of ammonia-N in the rumen. Energy supplementation for Nellore bulls on tropical pastures during the rainy season provides excess energy relative to protein, resulting in a substitution effect of supplement intake on forage intake. Moreover, energy supplementation does not improve body weight gain. Protein sources should be added to supplements for balancing protein and energy levels, thereby reducing a likely substitution effect.

The objectives of the present study were to evaluate the effect of heat load, bodyweight and dietary protein on oxygen pulse of heifers, to obtain the energy cost of walking on flat and sloping terrain, and to compare the energy cost of heifers during continuous walking and intermittent walking. In Experiment 1, the correlations of oxygen consumption, heart rate and oxygen pulse (O2P) with bodyweight, black globe temperature and temperature and humidity index were examined. Moreover, the effect of dietary protein on O2P was evaluated. The temperature and humidity index and black globe temperature had a low positive correlation with O2P and oxygen consumption, and a low negative correlation with heart rate. However, weight had no correlation with O2P. There was a linear increase in O2P with a very low adjustment with an increasing dietary crude protein concentration. In Experiment 2, the energy cost of heifers walking continuously at a constant speed in a terrain with 0%, 6% and 12% gradient was measured. The energy expenditure was similar among the terrain gradients. The heifers walking had a 16.6% higher energy expenditure than when they were standing. In Experiment 3, a comparison of the energy cost was made among heifers standing, continuously walking and intermittently walking at a constant speed on flat ground. The energy cost for walking was similar between continuous and intermittent walking. The heat load, bodyweight and dietary protein concentration had a low effect on O2P in dairy heifers. Therefore, measurements over a short time (5–15 min) are a reliable estimator of O2P through the day. Both intermittent and continuous walking can be used to evaluate energy expenditure.

Balancing diets for protein affects both protein and energy use efficiency in cattle. This study aimed to evaluate the effects of dietary protein level on intake, digestibility and energy expenditure in Holstein heifers. Four Holstein heifers with a mean BW of 266.5 ± 10.7 kg were distributed in a 4 x 4 balanced Latin square design. Each period was comprised of 14 d for adaptation and 6 d for samplings. The heifers were fed diets with 9, 12, 15 and 18% of crude protein (CP). Total feces and urine were collected. Rumen fluid was collected at 2, 4, 6 and 8 h after feeding. The energy expenditure was evaluated at 6 a.m., 12 a.m., 6 p.m. and 12 p.m. by a closed-circuit mask technique. The intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were similar between treatments (P > 0.05). Although the NDF digestibility was not changed (P > 0.05), the digestibilities of DM, OM, CP, non-fibrous carbohydrates, and total digestible nutrients increased linearly (P < 0.05) with increasing CP levels in the diet. Urinary urea nitrogen excretion and nitrogen retained increased linearly (P < 0.01) with increasing CP levels. Dietary CP levels did not affect (P =0.53) energy expenditure in heifers. Ruminal ammonia-nitrogen concentration peaked at 2-4 h after feeding. Increasing dietary CP levels from 9 to 18% does not affect feed intake and energy expenditure in heifers. However, it improves the digestibility of OM and nitrogen retention.