The study of the quantitative and qualitative composition of the soil microbiota, which adequately reflects the degree of anthropogenic load, can be applied as an indicator of assessment of the ecological status of the soil of anthropogenically transformed biogeocoenosis.The aim of the article is to analyze the species composition of micromycetes of drained soils of the Trubizh river Basin as an indicator of the ecological condition of the soil of different degrees of economic activity. For microbiological analysis samples of organogenic (lowland deep and medium peat soils) and mineral (meadow-chernozemic) soils were used. The samples selected from four experimental plots with different degrees of anthropogenic load. In particular, the first plot is a long-term use fallow (more than 15 years) on deep peatlands, the second one is a field with corn for grain on medium peatlands. The third plot is a field with post-harvest soybean residues on medium peatlands and fourth one is fallow with annual and perennial grasses on meadow-chernozemic soils. Soil samples were taken from the root layer at a depth of 0-5, 5-10 and 10-20 cm according to the current DSTU. Isolation of micromycetes was carried out by dilution method of soil with the object of estimation of the concentration (number of colonies, micromycetes). The count of micromycetes was estimated on surface of potato-glucose agar and Chapek's agar plates and was determined by the number of colony forming units (CFU). It was established that in the plot with increased anthropogenic impact (field with corn for grain on medium peatlands) the total number of isolated micromycetes was twice less than in plots of fallow lands and soybean. There was observed very poor species compositions (13 species). 2 species (15%) of Zygomycota, where predominant concentration was Mucor plumbeus in the soil layer 0-20 cm and 11 species (85%) of Ascomycota were found. In all plots, the basis of micromycete complexes was formed by fungi-destructors of plant remains and root secretions of the genus Penicillium. The micromycetes of drained soils of the Trubizh river Basin was presented the biggest phylum Ascomycota (83-95.5%) and the phylum Zygomycota (4.5-17%) also.

The problem of environmental safety of water bodies is relevant today, especially for the basins of small and medium-sized rivers, which are clear indicators of the environment state. One of them is the Trubizh River. The water resources of this river are formed under the anthropogenically changed conditions of the drainage and humidification complex of the Trubizh reclamation system. These resources are also receivers of effluents from point and diffuse sources. Thus, there is a need for constant monitoring of the ecological condition of the river. The water quality of it is a consequence of anthropogenic activities in the watershed. The aim of the work is a comprehensive assessment of the Trubizh River ecological condition under the modern conditions of water quality formation. The analysis has been conducted using official data from the state monitoring of water quality for 2015-2019 years and our own research for 2015-2016 years. 7 observation points have been considered from souse to mouth of the river: border areas of Brovary and Baryshivka; Baryshivka – 1 km above and 0.9 km below the village, Pereyaslav-Khmelnytsky – 0.5 km above and 1 km below the town, the mouth of the Alta and Trubizh rivers). We used such methods as analytical (determination of water quality indicators according to generally accepted standardized methods); analysis, systematization and generalization of the monitoring database; method of calculation of integrated block indices and complex ecological index of water quality (IE). It is established that during the five-year period (2015-2019) according to the weighted average and the worst values of the complex ecological index (IEworst 4.2) the waters are characterized as "satisfactory", "slightly polluted" of the III class quality 4 category. In 2018 and 2019 years, there was a deterioration in water quality by one category (III quality class 5 category) - "mediocre", "moderately polluted" water. This is due to a set of conditions of natural and climatic (insufficient rainfall and rising air temperature) and anthropogenic nature (water pollution, unauthorized surface water abstraction, runoff obstruction, etc.). It is noted that Trubizh river is characterized by stable uniform spatial water pollution. The largest values of IE are recorded in the area of influence of the village Baryshivka (IE worst 3.7-3.8) and Pereyaslav-Khmelnytskyi in the sampling points: the Alta river mouth (IEworst 4.0) and the Trubizh river mouth (IEworst 3.7). The water quality in these sampling points corresponded to class III of category 4 and was generally characterized as "satisfactory", "polluted". In general, the deterioration of water quality is caused by organic pollution (according to the indicator of dichromate oxidation (IV class, 6 category), BOD5 (Biochemical oxygen demand) (III class, 5 category), nitrogen compounds (ammonium, nitrite and nitrate) – V class, 7 category) and phosphorus compounds (phosphate ions – III class, 5 category), total iron and general chromium – III class, 5 category, phenols and SSAS (synthetic surfactants active substances) – IV class, 6 category. Thus, one of the main reasons for the degradation of the river is the anthropogenic conditionality of its development as a result of the urbanized and agricultural areas impact. The obtained data open the prospect of continuing work on monitoring and assessing the ecological status of aquatic ecosystems of the Trubizh River for the further development of scientifically based recommendations. It is necessary for making management decisions for sustainable use and protection of surface waters and restoration of aquatic ecosystems of Trubizh River Basin.