A study was conducted of the chemical effects on the human kidney induced by the chronic ingestion of uranium in drinking water. Subjects were divided into two groups: The low-exposure group, whose drinking water was obtained from a municipal water system and contained < 1 microgram uranium/L, and the high-exposure group, whose drinking water was obtained from private drilled wells and contained uranium levels that varied from 2 to 781 micrograms/L. Years of residence varied from 1 to 33 years in the low-exposure group and from 3 to 59 years in the high-exposure group. The indicators of kidney function measured in this study included glucose, creatinine, protein, and beta 2-microglobulin (BMG). The markers for cell toxicity studied were alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), and N-acetyl-beta-D-glucosaminidase (NAG). Urinary glucose was found to be significantly different and positively correlated with uranium intake for males, females, and pooled data. Increases in ALP and BMG were also observed to be correlated with uranium intake for pooled data. In contrast, the indicators for glomerular injury, creatinine and protein, were not significantly different between the two groups nor was their urinary excretion correlated to uranium intake. These results suggest that at the intakes observed in this study (0.004 microgram/kg to 9 micrograms/kg body wt), the chronic ingestion of uranium in drinking water affects kidney function and that the proximal tubule, rather than the glomerulus, is the site for this interference.

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The authors conducted a study of an aboriginal community to determine if kidney func-tion had been affected by the chronic ingestion of uranium in drinking water from the community's drilled wells. Uranium concentrations in drinking water varied from < 1 to 845 ppb. This nonin-vasive study relied on the measurement of a combination of urinary indicators of kidney function and markers for cell toxicity. In all, 54 individuals (12-73 years old) participated in the study. Correlation of uranium excreted in urine with bio-indicators at p

The gastrointestinal absorption factor (f1) for uranium in humans has been determined from a study of 50 volunteers, ingesting uranium at natural levels in drinking water and food. The purpose of the study was to find an appropriate f1 value for humans to use in deriving exposure guidelines for uranium. The participants ranged in age from 13 to 87 years. They were selected from two communities: New Ross, Nova Scotia with elevated uranium in drinking water, and Ottawa, Ontario with very low levels of uranium. Uranium intake and excretion were measured in samples collected concurrently from the same individuals over a three-day period. The duplicate diet method was used to monitor uranium intake in food and water. Uranium levels in all samples were measured by inductively coupled plasma mass spectrometry (ICP/MS). The distribution of f1 values obtained was non-Gaussian with a range of 0.001 to 0.06 and a median of 0.009. Seventy-eight percent of the subjects had values less than 0.02. These values are consistent with the recommendations of ICRP 69. The f1 values were not gender-sensitive and were independent of age at time of study, duration of exposure, and total uranium intake. The implications of these findings are discussed in terms of setting drinking water guidelines.

The Canadian National Calibration Reference Center for Bioassay and in-vivo Monitoring is part of the Radiation Protection Bureau, Department of Health. The Reference Center operates a variety of different intercomparison programs that are designed to confirm that workplace monitoring results are accurate and provide the necessary external verification required by the Canadian regulators. The programs administered by the Reference Center currently include urinalysis intercomparisons for tritium, natural uranium, and 14C, and in-vivo programs for whole-body, thorax, and thyroid monitoring. The benefits of the intercomparison programs to the participants are discussed by example. Future programs that are planned include dual spiked urine sample which contain both tritium and 14C and the in-vivo measurement of 99mTc.