BackgroundNatural products isolated from plant sources have been demonstrated as potential candidates against several ailments. The scientific investigations on the underlying principles of phytotherapy can pave way for the convergence of traditional medicines and modern science and technologies.ResultsQuercetin 3-O-(2′′-acetyl)-glucoside obtained from ethyl acetate fraction of Cleome viscosa is studied against inflammatory of carrageenan-induced rat paw edema ( in vivo) and microbial activity on ( in vitro). The structure of the glycoside is confirmed by means of hydrogen-1 nuclear magnetic resonance spectroscopy, carbon nuclear magnetic resonance spectroscopy, attached proton test, and mass spectrum. The flavonoid glycoside showed significant anti-inflammatory activity of on carrageenan-induced rat paw edema ( in vivo) and anti-microbial activity ( in vitro) on Staphylococcus aureus (gram positive) and Escherichia coli (gram negative). The anti-inflammatory effect of the flavonoid glycoside may be due to the inhibition of prostaglandin synthesis. Selective toxicity with flavonoid glycoside towards the gram-positive bacteria was found on S. aureus.ConclusionsThe present study reveals the anti-inflammatory and antimicrobial activities of an isolated quercetin 3-O-(2′′-acetyl)-glucoside from a natural source ( C. viscosa).

Aim:To analyze the antioxidants and anti-inflammatory potential of ethanolic crude extract of Cassia auriculata flowers. Study Design: 2 kg of fresh flowers were soaked with 90% ethanol at room temperature (25ºC-30ºC). After 72 hrs the ethanolic extract was filtered. This extract was distilled and finally the crude was obtained. This ethanolic crude to get required concentrations and were used for screening antioxidants and anti-inflammatory activities.

To assess the antioxidant and anti-inflammatory activities of the powder sample derived from the ethyl acetate fraction of the floral Argemone mexicana L. For antioxidant efficiency, the floral extract was evaluated using 1, 1–diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and the FRC (Ferric Reduction Capacity) assay. In vitro anti-inflammatory activity was assessed using human peripheral blood mononuclear cells (PBMC) induced by lipopolysaccharide (LPS) and the production method of nitric oxide (NO). The powder sample extracted from ethyl acetate fraction of the floral of Argemone Mexicana L showed good antioxidant activity with the comparative standard sample in scavenging DPPH radicals and in FRC assay. In the cell viability (PBMC influenced by LPS) method and the Nitric oxide (NO) assay, this sample showed even able anti-inflammative activities. Such results indicate a significant antioxidant and anti-inflammatory activity in the powder sample obtained from ethyl acetate fraction in the flower of Argemone mexicana L.

The yellow parts of the flowers of Asystasia gangetica were extracted with 85% methanol under reflux. The alcoholic extract was concentrated in vacuo and the aqueous extract was fractionated with solvents of increasing polarity. The ethyl acetate fraction yielded a yellow solid, which was characterized as apigenin 7Keywords: Asystasia gangetica, Acanthaceae, biflavone glycoside.Asystasia gangetica Linn. of the Acanthaceae family is an erect, procumbent herb found in peninsular India. It is cultivated in the garden as an ornamental plant. The leaves are good sources of thiamine [1]. The juice of the plant is used as an anthelmintic. It is also given in swellings and rheumatism [2,3]. The flowers are white or yellow in color. The yellow flowers have violet or pink coloration at the center of the flower. Previous phytochemical investigations of this plant showed the presence of flavonoids [4][5][6]. With the view to locate the flavonoids of yellow flowers of this plant, yellow parts of the flowers alone were chosen, and the results are presented hereunder. They were found to contain a biflavone glycoside.The yellow flowers of Asystasia gangetica were extracted and fractionated, and the ethyl acetate extract yielded a yellow solid 1. The UV spectrum of the glycoside had O max at 340 nm (band I) and 265 nm (band II), suggesting the presence of a flavone skeleton. In the NaOMe spectrum, a bathochromic shift of 45 nm was noticed. This indicates the presence of a 4c-OH group. No bathochromic shift was noticed in the NaOAc spectrum of the glycoside, whereas a bathochromic shift of 11 nm was noticed in the case of the aglycone. This shows the absence of free OH at C-7 in the glycoside and the presence of the same in the aglycone. Spectral changes with other shift reagents could not be identified because of the superimposition of bands. The presence of free OH at C-5 was evidenced by the positive Wilson's boric acid test.In the 1 H NMR spectrum (DMSO-d 6 , TMS) of the glycoside there are two signals, one at G 12.98 and another at G 12.95, indicating the presence of two chelated hydroxyl groups. These signals are assigned to OH groups present at C-5 and C-5cc, respectively. The C-6c proton appears as a doublet at G 7.9 due to ortho coupling with H-5c. The C-5c proton appears as a doublet at G 6.9 due to ortho coupling with H-6c. The C-2c proton that is ortho to the interflavonoid linkage experiences an upfield shift and appears at G 7.62. The C-6 proton appears at G 6.44 ppm, and the C-8 proton appears as a doublet at G 6.86 due to meta coupling with H-6. The C-8cc and C-3cc protons appear as singlets at G 6.78 and G 6.74, respectively. The C-2ccc and C-6ccc protons appear at G 7.45 and G 7.42, respectively. The C-5ccc proton appears as a doublet at G 6.94 due to ortho coupling with H-6ccc (7.45) as observed in the 1 H-1 H COSY spectrum. The two doublets due to anomeric protons of the glucose units attached to the C-7 and C-7cc carbons merge at G 5.1. 13 C NMR (DMSO-d 6 , TMS) spectral data provide supporting evidence for th...

No abstract