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Genetic variability, correlation and path analysis was carried out for yield and yield components in 542 F 3 progeny lines developed by crossing JK8 x Peddasame (purple late) in little millet during Kharif 2015. Seven biometrical characters were studied for estimating genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), genetic advance, heritability (broad sense), genetic advance as per cent of mean, correlation coefficient and path coefficient among themselves. High GCV and PCV were observed for number of productive tillers per plant and per plant yield. High heritability was observed for plant height and 1000 seed weight. Number of productive tillers per plant showed maximum genetic advance as per cent of mean followed by grain yield per plant, panicle length and plant height. High heritability coupled with moderate genetic advance as per cent of mean were observed for plant height and 1000 seed weight. Grain yield per plant possessed significant positive correlation with plant height, panicle length, number of productive tillers per plant and 1000 seed weight. Number of productive tillers per plant imparted maximum direct effect on grain yield followed by panicle length, 1000 seed weight and plant height.

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Clusterbean seed health testing is warranted since the pathogen (Xanthomonas campestris pv. cyamopsidis (Xccy)) is seed-borne and seed-transmitted. A polyclonal antibody was developed in rabbit via subcutaneous and intramuscular injections and characterized for sensitivity, specificity and its applicability to ELISA which: (i) was sensitive in detecting as few as 10 2 cells ml −1 at a titre of 1: 4000; (ii) was specific, since it reacted only with Xccy and not with other xanthomonads; (iii) reacted both with Xccy cells and culture filtrate, indicating that the antigenic determinant is a secretory component; (iv) was applicable and reliable in seed health testing since it reacted only with infected seeds and plant materials and not with healthy seeds and (v) a purified fraction of antibody was virulent-specific since heat-denatured and avirulent isolates were not detected. The ELISA thus developed is highly reproducible and therefore suitable for the evaluation of the potential disease status of seeds and plant health, which is appropriate for routine seed health testing.

The present study was focused on wild pantropical macrofungi, Macrocybe gigantea (Massee) Pegler & Lodge, collected from Agumbe rain forests of Karnataka, India. The macrofungal tissues was subjected to mycelial culturing in four different solid nutritive media viz., Potato Dextrose Agar, Malt Extract Agar, Sabouraud's Dextrose Agar and Czapek Dextrose Agar. The study also evaluated the invitro antibacterial activity of ethyl acetate extract of mycelial filtrates obtained from Potato Dextrose Broth cultures of M. gigantea. The crude extracts were tested against five Gram-negative bacteria using agar well diffusion assay. Our data showed that among the soild media tested, the mycelium grown in Potato Dextrose Agar recorded the maximum growth followed by Malt Extract Agar. The crude extracts had inhibition against Salmonella typhi, Escherihia coli, Klebsiella pneumoniae, Pseudomonas syringae and Xanthomonas campestris. The maximum inhibition was against S.typhi. The results revealed that the extracts had the potent bioactive compounds with antibacterial property.