The study was conducted in Khulna, Satkhira and Jhenaidah districts under Khulna division. The purpose was to know the training of fishery officers, fish and shrimp farmers in aqua farming. The main dissemination processes are training of farmers, farm demonstration, farmer to farmer visits, leaflets and pamphlets, audiovisuals etc. In Khulna district dissemination and extension services are application of government rules and regulations, strategic management, pond/shrimp farm visit, public awareness, training facilities, financial and other supports and some research projects also applied, training program, the number of trained fish farmers and trained shrimp farmers in 2009 were 790 and 5,754 respectively. In Satkhira district, different extension programs are applied, such as shrimp demonstration, fish sanctuary, pond/shrimp farm visit, training program, the number of trained Fish farmers and trained shrimp farmers in 2009 were 934 and 2110 respectively and community based fisheries management and some research projects. In Jhenaidah district DoF activities are training, in sadar upazila number of trained fisherman 34, baor management, identify floodplain and other involved communities, awareness campaign, frame survey, arrangement of fund, credit facilities, training program, production monitoring, identification of disadvantaged groups and pollution, disease diagnostic and its control.

Introduction: This study was conducted to detect the presence of enterovirulent Escherichia coli strains using PCR (polymerase chain reaction) technique in some selected unhygienic and hygienic traditional shrimp farms in Bangladesh. The target genes chosen for this investigation included: the phoa housekeeping gene (present in all E. coli); the lt1, lt2 and st1 genes of ETEC (enterotoxigenic); the vtverotoxin, and eae virulence genes of EHEC (enterohaemorrhagic) and EPEC (enteropathogenic), respectively. Methods: Six pairs of oligonucleotide primers were designed to amplify internal fragments of these genes by PCR to generate PCR products that were analyzed by gel electrophoresis. The presence of E. coli strains were determined by visualization of strain specific band comparing with a 100 bp DNA size marker in UV transilluminator. Results:The results revealed that water of both hygienic and unhygienic farms were highly contaminated with E. coli followed by sediment and shrimp. Whereas, only water, sediment and shrimp of unhygienic farms were found to be contaminated with virulent E. coli strains. Conclusions: Among the virulent E. coli strains, ETEC and EPEC strains were detected in unhygienic farms, but no EHEC strain was detected. However, no contamination with virulent E. coli strains was found in hygienic farms.