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The Internet of Things is quickly becoming one of the most important developments in this century's automation technology field. The Internet of Things (IoT) establishes a new approach to resolving issues that may arise in the foreseeable future by establishing an interface between the many devices that are now in use and integrating the functionalities of these devices. This study's primary objective is to develop methods that will cleverly simplify living arrangements and increase their convenience. The majority of things are now automated and computerized. This work aims to provide a system for integrating sensors and other data sources for different home automation. A home with intelligence on par with humans is called a smart home. This study proposes an IoT-based smart home automation system that operates in different modules to automate the complete home. If there is a problem, it will immediately identify and attempt to resolve it. In that case, it will send a notification to the homeowner. The suggested prototype included an automated door lock system, an automated gas detection system, and a smart smoke and fire alert system that sends an alert message to the owner and remotely controls the inside fan light. The system will immediately notify the users if a fire is detected in the user's residence. A smartphone application known as the Blynk App controls things remotely. As a result of being transformed into a smart home, it can offer security, energy efficiency, and the capability to control all of the current appliances in the house remotely or from a central place. Due to the exponential growth of technology, day-to-day activities are becoming less complicated, which has contributed to the popularity of home automation systems in recent years.

Introduction: This study was conducted to detect the presence of enterovirulent Escherichia coli strains using PCR (polymerase chain reaction) technique in some selected unhygienic and hygienic traditional shrimp farms in Bangladesh. The target genes chosen for this investigation included: the phoa housekeeping gene (present in all E. coli); the lt1, lt2 and st1 genes of ETEC (enterotoxigenic); the vtverotoxin, and eae virulence genes of EHEC (enterohaemorrhagic) and EPEC (enteropathogenic), respectively. Methods: Six pairs of oligonucleotide primers were designed to amplify internal fragments of these genes by PCR to generate PCR products that were analyzed by gel electrophoresis. The presence of E. coli strains were determined by visualization of strain specific band comparing with a 100 bp DNA size marker in UV transilluminator. Results:The results revealed that water of both hygienic and unhygienic farms were highly contaminated with E. coli followed by sediment and shrimp. Whereas, only water, sediment and shrimp of unhygienic farms were found to be contaminated with virulent E. coli strains. Conclusions: Among the virulent E. coli strains, ETEC and EPEC strains were detected in unhygienic farms, but no EHEC strain was detected. However, no contamination with virulent E. coli strains was found in hygienic farms.

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