Microalgae is a very potential to be developed biota Because of its abundant amount on earth. Research on microalgae and its application as pigment analysis, provides important information about chemical compounds such as carotenoids and chlorophyll roomates that can be developed into drugs. Microalgae such as Chlorella vulgaris, Nannochloropsis sp., Porphyridium cruentum and Spirulina platensis has known had carotenoid pigments and chlorophyll content, but the optimization of the production of pigment in microalgae life phase has not been known in detail. Chlorophyll content in microalgae in the logarithmic phase of Chlorella vulgaris 200-1500 mg/L, Nannochloropsis sp. 100-500 g/L, Porphyridium cruentum 500-800 g/L and Spirulina platensis 1000-3500 mg/L and for stationary phases microalgae Chlorella vulgaris 100-1000 mg/L, Nannochloropsis sp. 200-500 g/L, Porphyridium cruentum 900-2000 mg/L and Spirulina platensis 2000-6000 mg/L. While the carotenoid content of microalgae in the logarithmic phase of Chlorella vulgaris 10-40 g/L, Nannochloropsis sp. 10-60 g/L, Porphyridium cruentum 10-60 ug/L and Spirulina platensis 20-40 ug/L and for stationary phases microalgae Chlorella vulgaris 10-50 g/L, Nannochloropsis sp. 10-70 g/L, Porphyridium cruentum 70-130 ug/L and Spirulina platensis 20-1100 mg/L.

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