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The iso-intensity line curvature algorithm in laser ektacytometry of red blood cells is investigated by numerical simulation. The algorithm is designed to measure the average deformability, as well as the width and asymmetry of the red blood cell deformability distribution in a blood sample under study. The accuracy and scope of the algorithm are determined. Using a bimodal ensemble as an example, the possibility of determining the fraction of weakly deformable red blood cells in a blood sample by laser ektacytometry is demonstrated.

The problem of measuring the deformability of erythrocytes by laser diffractometry in shear flow (ektacytometry) is considered. The question of how the shape of the isointensity line in the diffraction pattern, which arises when a laser beam is scattered by an ensemble of erythrocytes, is related to the level of light intensity on this line, is analyzed. A simplified algorithm for measuring the parameters of the distribution of erythrocytes in deformability is proposed, which does not require photometry of the central part of the diffraction pattern.

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The study describes an analytical model of laser beam scattering on an inhomogeneous ensemble of erythrocytes. The model takes into account erythrocyte variation in size, shape, and spatial orientation. A relationship is established between an experimentally determined parameter: visibility of the diffraction pattern, and a characteristic serving as a measure of inhomogeneity of erythrocyte size and shape in a blood specimen. This relationship is shown to be monotonous, which means that erythrocyte variation in size and shape can be assessed based on measurements of the diffraction pattern visibility.