his study was designed to investigate the effect of inoculation time with vesicular arbuscular mycorrhizal (VAM) fungi, Glomus spp., on the interaction between root-rot and stem-rot of pepper caused by Sclerotium rolfsii Sacc. as well as its growth. The results indicated that addition of Glomus spp. significantly increased the shoot and root fresh and dry weight with significant decrease to the disease severity (59-89%). Effect of the mycorrhizal inoculation using five species of Glomus by two different inoculation treatments on incidence of Sclerotium stem-rot as well as crop parameters of pepper plants inoculated with the tested fungus was investigated. Generally, mycorrhizal inoculation increased pepper shoot and root fresh and dry weight compared with uninoculated plants. Meanwhile, seedlings arose from seeds treated with Glomus spp. increased growth parameters and yield. G. coronatum and G. luteum were the best mycorrhizae to increase crop parameters. On the other hand, G. etunicatum, G. diaphanum and G. luteum were the best to protect pepper plants against S. rolfsii.
A large number of diseases has been reported to attack pepper plants in Egypt; of these, damping -off caused by Sclerotium rolfsii Sacc. (The sclerotial state of Athelia rolfsii Curtz) is the most common. Four fungi, i.e. Fusarium solani Mart, Fusarium oxysporum Schlecht, S. rolfsii and Rhizoctonia solani Kuhn were isolated from rotted roots of pepper plants. Pathogenicity test indicated that the highest percentages of pre-and post-emergence damping -off were recorded on pepper and tomato plants sown in soil infested with S. rolfsii, R. solani, and F. solani. In vitro studies showed that Trichoderma harzianum Rifai and Bacillus subtilis Ehrenberg caused clear inhibition to S. rolfsii growth. In vivo studies, treatment of pepper seeds with either T. harzianum or B. subtilis showed a significant decrement in the infection of pepper plants by S. rolfsii. Some fungicides, i.e., Vitavax-Thiram, Rizolex T, Tashgarin and Moncerin completely inhibited the growth of S. rolfsii at 100 ppm concentration. In vivo studies, treatment of pepper seeds with any of the aforementioned fungicides at the recommended dose caused significant decrement in the infection of pepper plants by S. rolfsii.
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