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The determination of antibodies against cytomegalovirus-induced antigens in 30 patients with acute cytomegalovirus infection and in 100 healthy control persons, using early antigen preparations of human lung fibroblasts and of rabbit lung fibroblasts, yielded results showing a high degree of agreement. For such investigations, however, the use of rabbit cells proved to be of advantage because the preparation of the early antigens can be carried out without inhibition of the DNA synthesis of the host cells.