The effect of CaUistemon lanceolatus (Syn. C. citrinus curtis; Family: Myrtaceae) leaf oil was studied for the antinociceptive and anti-inflammatory activity in experimental animals. C. lanceolatus, 25-100 mgkg administered orally for 3 days exhibited graded dose response equivalent to 21.95%-89.90% protection in the tail flick latent test in rat. The C. lanceolatus oil (50 and 100 mgkg, given orally for 3 days) was effective in hot plate reaction time (64.05% and 112.97%, p< 0.01 and p< 0.001), analgesymeter induced mechanical pain (28.17% and 54.42%, p C 0.01 and p C 0.001) and acetic acid-induced writhing (26.68% and 51.79%, p < 0.5 and p < 0.05) in mice. The oil of C. lanceolatus potentiated the analgesic activity with pentazocine (10 mgkg, i.p.) and aspirin (25 mgkg, i.p.). In the carrageenan-induced paw edema C. lanceolatus oil (50 and 100 mglkg, given orally for 3 days) decreased paw volume significantly (26.68% and 51.79%) and dose dependent anti-inflammatory activity in 1-3 hour time interval and potentiated with nimesulide (50 mgkg, p.0.). In summary, this study demonstrates that leaf oil of C. lanceolatus has significant antinociceptive and anti-inflammatory activity.