We have recently isolated a biofilm-producing strain (C208) of Rhodococcus ruber that degraded polyethylene at a rate of 0.86% per week (r2=0.98). Strain C208 adheres to polyethylene immediately upon exposure to the polyolefin. This initial biofilm differentiates (in a stepwise process that lasts about 20 h) into cell-aggregation-forming microcolonies. Further organization yields "mushroom-like" three-dimensional structures on the mature biofilm. The ratio between the population densities of the biofilm and the planktonic C208 cells after 10 days of incubation was about 60:1, indicating a high preference for the biofilm mode of growth. Analysis of extracellular polymeric substances (EPS) in the biofilm of C208 revealed that the polysaccharides level was up to 2.5 folds higher than that of the protein. The biofilm showed a high viability even after 60 days of incubation, apparently due to polyethylene biodegradation.
A model of gas metal arc welding was developed that solves the magnetohydrodynamic equations for the flow and temperature fields of the molten electrode and plasma simultaneously, to form a fully coupled model. A commercial finite-element code was extended to include the effects of radiation, Lorentz forces, Joule heating and thermo-electric effects. The model predicts the shape of the free surfaces of the molten metal as the droplets form, detach, and merge with the weld pool. It also predicts the flow, temperature, and electric field. Material properties and the welding parameters are the input variables in the model. The geometry of the numerical model was constructed to fit an experimental apparatus using an aluminium electrode and an argon shielding gas. Droplet frequency measurements were used to verify the model's predictions. For a typical arc, the temperature of the plasma can range up to 20 000 K, where there is more uncertainty in the thermophysical properties of the plasma, and the properties in this range are highly non-linear. For this range, the material properties of the model were adjusted to obtain a better fit between the numerical and the experimental results. The model and experimental results were comparable.
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