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Aim: To study the chromium picolinate mediated with zinc oxide nanoparticle and its cytotoxicity and antimicrobial activity Introduction: Chromium can alleviate glucose intolerance and insulin resistance and it is involved in the metabolism of glucose, lipid, protein, and nucleic acid .Chromium picolinate [CrPic], also named as picolinic acid chromium and several studies have proved that CrPic, as the source of Chromium, can alleviate the high level of blood glucose, blood lipid, insulin, and cholesterol in the patients with metabolic syndrome and  zinc oxide [ZnO] has also gained momentum due to their unique properties in the process of nanotechnology. Chromium picolinate works together with insulin that produced by the pancreas to metabolize carbohydrates. It's made by combining chromium with picolinic acid. The acid helps the body absorb chromium. Randomized controlled trials have failed to demonstrate a link between chromium supplementation and the prevention or treatment of type 2 diabetes or impaired glucose tolerance. Chromium supplementation of young men and women does not promote muscle accretion, fat loss, or gains in strength. Physically active individuals with concerns about meeting guidelines for nutrient intake should be counseled to select and consume foods with high nutrient densities rather than to rely on nutritional supplements. Materials and Methods: Chromium picolinate mediated with ZnO nanoparticles were evaluated for its antibacterial activity and cytotoxicity potential using brine shrimp lethality assay. Results: Chromium picolinate mediated zinc oxide nanoparticles show good results in antimicrobial activity as well as in cytotoxicity. Conclusion: Chromium picolinate mediated Zn nanoparticles is an efficient antibacterial and a potential cytotoxicity agent.

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